Modulation by opioid peptides of mechanosensory pathways supplying the guinea-pig inferior mesenteric ganglion

R. C. Ma, J. H. Szurszewski

Research output: Contribution to journalArticle

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Abstract

1. Radioimmunological techniques were used in isolated guinea-pig inferior mesenteric ganglion (IMG)-colon preparations to determine whether opioid peptides and neurotensin8-13 (NT8-13), the C-terminal region of NT1-13 recognized by neurotensin receptors, modulate distension-induced release of substance P (SP)- and vasoactive intestinal polypeptide (VIP)-like immunoreactive (LI) material. 2. Colonic distension significantly increased the amount of SP- and VIP-LI material released in the ganglionic superfusate. A low-Ca2+ (0.1 mM), high-Mg2+ (15 mM) solution blocked their release. 3. In vivo capsaicin pretreatment abolished release of SP-LI material during colonic distension but had no significant effect on distension-induced release of VIP-LI material. 4. The addition of (Leu5]enkephalin, [Met5]enkephalin, PL017 (a μ-receptor agonist) and DPDPE (a δ-receptor agonist) to the ganglion side of a two-compartment chamber blocked distension-induced release of SP-LI material. The addition of naloxone and ICI-174,864 (a δ-receptor antagonist) to the ganglion compartment reversed the inhibitory effect of the μ- and δ-receptor agonists. 5. Addition of [Leu5]enkephalin and [Met5]enkephalin to the ganglion compartment had no significant effect on release of VIP-LI material during colonic distension. 6. Addition of NT8-13 to the ganglion compartment significantly increased in the amount of SP-LI material released during colonic distension but had no affect on distension-induced release of VIP-LI material. 7. The results suggest the hypothesis that under in vivo conditions, enkephalinergic nerves decrease and neurotensinergic nerves increase the release of SP from peripheral branches of primary afferent sensory nerves.

Original languageEnglish (US)
Pages (from-to)435-445
Number of pages11
JournalJournal of Physiology
Volume491
Issue number2
StatePublished - Mar 1 1996

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Opioid Peptides
Substance P
Ganglia
Vasoactive Intestinal Peptide
Guinea Pigs
Enkephalins
Neurotensin Receptors
D-Penicillamine (2,5)-Enkephalin
Capsaicin
Naloxone
Colon

ASJC Scopus subject areas

  • Physiology

Cite this

Modulation by opioid peptides of mechanosensory pathways supplying the guinea-pig inferior mesenteric ganglion. / Ma, R. C.; Szurszewski, J. H.

In: Journal of Physiology, Vol. 491, No. 2, 01.03.1996, p. 435-445.

Research output: Contribution to journalArticle

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