Modified intranuclear organization of regulatory factors in human acute leukemias: Reversal after treatment

Jeffrey A. Gordon, Shirwin M. Pockwinse, F. Marc Stewart, Peter J. Quesenberry, Tatsuya Nakamura, Carlo M. Croce, Jane B. Lian, Janet L. Stein, Andre J van Wijnen, Gary S. Stein

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Acute leukemias arise secondary to chromosomal aberrations that cause dysfunctions in gene regulation and regulatory factors. Significant differences in morphology between acute leukemic and nonleukemic hematopoietic cells are readily observed. How morphologic changes of the nuclei of acute leukemic cells relate to the underlying functional alterations of gene expression is minimally understood. Spatial modifications in the representation and/or organization of regulatory factors may be functionally linked to perturbations of gene expression in acute leukemic cells. Using in situ immunofluorescence microscopy, we addressed the interrelationships of modifications in nuclear morphology with the intranuclear distribution of leukemia-related regulatory factors (including ALL-1, PML, and AF-9) in cells from patients with acute leukemia. We compared the localization of leukemia-associated proteins with various factors involved in gene transcription and RNA processing (e.g., RNA polymerase II and SC-35). Our findings suggest that there are leukemia-associated aberrations in mechanisms that direct regulatory factors to sites within the nucleus. This misplacement of key cognate factors may contribute to perturbations in gene expression characteristic of leukemias. (C) 2000 Wiley- Liss, Inc.

Original languageEnglish (US)
Pages (from-to)30-43
Number of pages14
JournalJournal of Cellular Biochemistry
Volume77
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Gene expression
Leukemia
Aberrations
Gene Expression
RNA Polymerase II
Transcription
Therapeutics
Microscopic examination
Genes
RNA
Regulator Genes
Fluorescence Microscopy
Chromosome Aberrations
Processing
Proteins

Keywords

  • Acute lymphocytic leukemia (ALL)
  • Acute myelogenous leukemia (AML)
  • Cancer
  • Gene expression
  • Promyelocytic leukemia (PML)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Modified intranuclear organization of regulatory factors in human acute leukemias : Reversal after treatment. / Gordon, Jeffrey A.; Pockwinse, Shirwin M.; Stewart, F. Marc; Quesenberry, Peter J.; Nakamura, Tatsuya; Croce, Carlo M.; Lian, Jane B.; Stein, Janet L.; van Wijnen, Andre J; Stein, Gary S.

In: Journal of Cellular Biochemistry, Vol. 77, No. 1, 2000, p. 30-43.

Research output: Contribution to journalArticle

Gordon, JA, Pockwinse, SM, Stewart, FM, Quesenberry, PJ, Nakamura, T, Croce, CM, Lian, JB, Stein, JL, van Wijnen, AJ & Stein, GS 2000, 'Modified intranuclear organization of regulatory factors in human acute leukemias: Reversal after treatment', Journal of Cellular Biochemistry, vol. 77, no. 1, pp. 30-43. https://doi.org/10.1002/(SICI)1097-4644(20000401)77:1<30::AID-JCB4>3.0.CO;2-K
Gordon, Jeffrey A. ; Pockwinse, Shirwin M. ; Stewart, F. Marc ; Quesenberry, Peter J. ; Nakamura, Tatsuya ; Croce, Carlo M. ; Lian, Jane B. ; Stein, Janet L. ; van Wijnen, Andre J ; Stein, Gary S. / Modified intranuclear organization of regulatory factors in human acute leukemias : Reversal after treatment. In: Journal of Cellular Biochemistry. 2000 ; Vol. 77, No. 1. pp. 30-43.
@article{bf63d09923504ba2815eafc86c88515f,
title = "Modified intranuclear organization of regulatory factors in human acute leukemias: Reversal after treatment",
abstract = "Acute leukemias arise secondary to chromosomal aberrations that cause dysfunctions in gene regulation and regulatory factors. Significant differences in morphology between acute leukemic and nonleukemic hematopoietic cells are readily observed. How morphologic changes of the nuclei of acute leukemic cells relate to the underlying functional alterations of gene expression is minimally understood. Spatial modifications in the representation and/or organization of regulatory factors may be functionally linked to perturbations of gene expression in acute leukemic cells. Using in situ immunofluorescence microscopy, we addressed the interrelationships of modifications in nuclear morphology with the intranuclear distribution of leukemia-related regulatory factors (including ALL-1, PML, and AF-9) in cells from patients with acute leukemia. We compared the localization of leukemia-associated proteins with various factors involved in gene transcription and RNA processing (e.g., RNA polymerase II and SC-35). Our findings suggest that there are leukemia-associated aberrations in mechanisms that direct regulatory factors to sites within the nucleus. This misplacement of key cognate factors may contribute to perturbations in gene expression characteristic of leukemias. (C) 2000 Wiley- Liss, Inc.",
keywords = "Acute lymphocytic leukemia (ALL), Acute myelogenous leukemia (AML), Cancer, Gene expression, Promyelocytic leukemia (PML)",
author = "Gordon, {Jeffrey A.} and Pockwinse, {Shirwin M.} and Stewart, {F. Marc} and Quesenberry, {Peter J.} and Tatsuya Nakamura and Croce, {Carlo M.} and Lian, {Jane B.} and Stein, {Janet L.} and {van Wijnen}, {Andre J} and Stein, {Gary S.}",
year = "2000",
doi = "10.1002/(SICI)1097-4644(20000401)77:1<30::AID-JCB4>3.0.CO;2-K",
language = "English (US)",
volume = "77",
pages = "30--43",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Modified intranuclear organization of regulatory factors in human acute leukemias

T2 - Reversal after treatment

AU - Gordon, Jeffrey A.

AU - Pockwinse, Shirwin M.

AU - Stewart, F. Marc

AU - Quesenberry, Peter J.

AU - Nakamura, Tatsuya

AU - Croce, Carlo M.

AU - Lian, Jane B.

AU - Stein, Janet L.

AU - van Wijnen, Andre J

AU - Stein, Gary S.

PY - 2000

Y1 - 2000

N2 - Acute leukemias arise secondary to chromosomal aberrations that cause dysfunctions in gene regulation and regulatory factors. Significant differences in morphology between acute leukemic and nonleukemic hematopoietic cells are readily observed. How morphologic changes of the nuclei of acute leukemic cells relate to the underlying functional alterations of gene expression is minimally understood. Spatial modifications in the representation and/or organization of regulatory factors may be functionally linked to perturbations of gene expression in acute leukemic cells. Using in situ immunofluorescence microscopy, we addressed the interrelationships of modifications in nuclear morphology with the intranuclear distribution of leukemia-related regulatory factors (including ALL-1, PML, and AF-9) in cells from patients with acute leukemia. We compared the localization of leukemia-associated proteins with various factors involved in gene transcription and RNA processing (e.g., RNA polymerase II and SC-35). Our findings suggest that there are leukemia-associated aberrations in mechanisms that direct regulatory factors to sites within the nucleus. This misplacement of key cognate factors may contribute to perturbations in gene expression characteristic of leukemias. (C) 2000 Wiley- Liss, Inc.

AB - Acute leukemias arise secondary to chromosomal aberrations that cause dysfunctions in gene regulation and regulatory factors. Significant differences in morphology between acute leukemic and nonleukemic hematopoietic cells are readily observed. How morphologic changes of the nuclei of acute leukemic cells relate to the underlying functional alterations of gene expression is minimally understood. Spatial modifications in the representation and/or organization of regulatory factors may be functionally linked to perturbations of gene expression in acute leukemic cells. Using in situ immunofluorescence microscopy, we addressed the interrelationships of modifications in nuclear morphology with the intranuclear distribution of leukemia-related regulatory factors (including ALL-1, PML, and AF-9) in cells from patients with acute leukemia. We compared the localization of leukemia-associated proteins with various factors involved in gene transcription and RNA processing (e.g., RNA polymerase II and SC-35). Our findings suggest that there are leukemia-associated aberrations in mechanisms that direct regulatory factors to sites within the nucleus. This misplacement of key cognate factors may contribute to perturbations in gene expression characteristic of leukemias. (C) 2000 Wiley- Liss, Inc.

KW - Acute lymphocytic leukemia (ALL)

KW - Acute myelogenous leukemia (AML)

KW - Cancer

KW - Gene expression

KW - Promyelocytic leukemia (PML)

UR - http://www.scopus.com/inward/record.url?scp=17944401552&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17944401552&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4644(20000401)77:1<30::AID-JCB4>3.0.CO;2-K

DO - 10.1002/(SICI)1097-4644(20000401)77:1<30::AID-JCB4>3.0.CO;2-K

M3 - Article

C2 - 10679814

AN - SCOPUS:17944401552

VL - 77

SP - 30

EP - 43

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 1

ER -