Abstract
The estimated S-phase fraction (%S) of non-Hodgkin's lymphomas has been demonstrated to be of prognostic value. The %S can be determined by labeling index (LI) techniques or by various mathematical models applied to DNA content histograms. We performed DNA content analysis and a slide-based bromodeoxyuridine immunofluorescence LI on split samples from 117 biopsy specimens suspicious for lymphoma. The LI was compared with the %S determined by two computer models (rectangular and polynomial) with and without debris subtraction, and a manual gates computer model. In the 93 DNA diploid cases, the rectangular models had the highest correlation with the LI (R=0.88). In the 24 aneuploid cases, the manual gates model was the most useful because of a high correlation with the LI (R=0.70) and its ability to be used in most cases (23/24). The polynomial models had limited usefulness because they generally gave a higher %S than the LI and could be fitted in less than half of the DNA aneuploid histograms. These results suggest that in situations where the LI is not possible, an accurate %S estimate can usually be obtained with either a rectangular or manual gates model.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 177-183 |
| Number of pages | 7 |
| Journal | Hematologic Pathology |
| Volume | 5 |
| Issue number | 4 |
| State | Published - 1991 |
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ASJC Scopus subject areas
- Hematology
- Pathology and Forensic Medicine
Cite this
Models of S-phase determination in lymphomas from flow cytometric DNA content histograms : Comparison with the bromodeoxyuridine labeling index. / Beauregard, P.; Witzig, Thomas Elmer; Kurtin, P. J.; Greipp, P. R.; Stenson, M. J.; Katzmann, J. A.; Habermann, Thomas Matthew; Wieand, H. S.
In: Hematologic Pathology, Vol. 5, No. 4, 1991, p. 177-183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Models of S-phase determination in lymphomas from flow cytometric DNA content histograms
T2 - Comparison with the bromodeoxyuridine labeling index
AU - Beauregard, P.
AU - Witzig, Thomas Elmer
AU - Kurtin, P. J.
AU - Greipp, P. R.
AU - Stenson, M. J.
AU - Katzmann, J. A.
AU - Habermann, Thomas Matthew
AU - Wieand, H. S.
PY - 1991
Y1 - 1991
N2 - The estimated S-phase fraction (%S) of non-Hodgkin's lymphomas has been demonstrated to be of prognostic value. The %S can be determined by labeling index (LI) techniques or by various mathematical models applied to DNA content histograms. We performed DNA content analysis and a slide-based bromodeoxyuridine immunofluorescence LI on split samples from 117 biopsy specimens suspicious for lymphoma. The LI was compared with the %S determined by two computer models (rectangular and polynomial) with and without debris subtraction, and a manual gates computer model. In the 93 DNA diploid cases, the rectangular models had the highest correlation with the LI (R=0.88). In the 24 aneuploid cases, the manual gates model was the most useful because of a high correlation with the LI (R=0.70) and its ability to be used in most cases (23/24). The polynomial models had limited usefulness because they generally gave a higher %S than the LI and could be fitted in less than half of the DNA aneuploid histograms. These results suggest that in situations where the LI is not possible, an accurate %S estimate can usually be obtained with either a rectangular or manual gates model.
AB - The estimated S-phase fraction (%S) of non-Hodgkin's lymphomas has been demonstrated to be of prognostic value. The %S can be determined by labeling index (LI) techniques or by various mathematical models applied to DNA content histograms. We performed DNA content analysis and a slide-based bromodeoxyuridine immunofluorescence LI on split samples from 117 biopsy specimens suspicious for lymphoma. The LI was compared with the %S determined by two computer models (rectangular and polynomial) with and without debris subtraction, and a manual gates computer model. In the 93 DNA diploid cases, the rectangular models had the highest correlation with the LI (R=0.88). In the 24 aneuploid cases, the manual gates model was the most useful because of a high correlation with the LI (R=0.70) and its ability to be used in most cases (23/24). The polynomial models had limited usefulness because they generally gave a higher %S than the LI and could be fitted in less than half of the DNA aneuploid histograms. These results suggest that in situations where the LI is not possible, an accurate %S estimate can usually be obtained with either a rectangular or manual gates model.
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M3 - Article
C2 - 1794967
AN - SCOPUS:0026354032
VL - 5
SP - 177
EP - 183
JO - Hematologic Pathology
JF - Hematologic Pathology
SN - 0886-0238
IS - 4
ER -