Models of S-phase determination in lymphomas from flow cytometric DNA content histograms: Comparison with the bromodeoxyuridine labeling index

P. Beauregard, T. E. Witzig, P. J. Kurtin, P. R. Greipp, M. J. Stenson, J. A. Katzmann, T. M. Habermann, H. S. Wieand

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The estimated S-phase fraction (%S) of non-Hodgkin's lymphomas has been demonstrated to be of prognostic value. The %S can be determined by labeling index (LI) techniques or by various mathematical models applied to DNA content histograms. We performed DNA content analysis and a slide-based bromodeoxyuridine immunofluorescence LI on split samples from 117 biopsy specimens suspicious for lymphoma. The LI was compared with the %S determined by two computer models (rectangular and polynomial) with and without debris subtraction, and a manual gates computer model. In the 93 DNA diploid cases, the rectangular models had the highest correlation with the LI (R=0.88). In the 24 aneuploid cases, the manual gates model was the most useful because of a high correlation with the LI (R=0.70) and its ability to be used in most cases (23/24). The polynomial models had limited usefulness because they generally gave a higher %S than the LI and could be fitted in less than half of the DNA aneuploid histograms. These results suggest that in situations where the LI is not possible, an accurate %S estimate can usually be obtained with either a rectangular or manual gates model.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalHematologic Pathology
Volume5
Issue number4
StatePublished - Dec 1 1991

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Hematology

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