Modeling structural and functional deficiencies of RBM20 familial dilated cardiomyopathy using human induced pluripotent stem cells

Saranya P. Wyles, Xing Li, Sybil C. Hrstka, Santiago Reyes, Saji Oommen, Rosanna Beraldi, Jessica Edwards, Andre Terzic, Timothy Mark Olson, Timothy J Nelson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Dilated cardiomyopathy (DCM) is a leading cause of heart failure. In families with autosomal-dominant DCM, heterozygous missense mutations were identified in RNA-binding motif protein 20 (RBM20), a spliceosome protein induced during early cardiogenesis. Dermal fibroblasts from two unrelated patients harboring an RBM20 R636S missense mutation were reprogrammed to human induced pluripotent stem cells (hiPSCs) and differentiated to beating cardiomyocytes (CMs). Stagespecific transcriptome profiling identified differentially expressed genes ranging from angiogenesis regulator to embryonic heart transcription factor as initial molecular aberrations. Furthermore, gene expression analysis for RBM20-dependent splice variants affected sarcomeric (TTN and LDB3) and calcium (Ca2+) handling (CAMK2D and CACNA1C) genes. Indeed, RBM20 hiPSCCMs exhibited increased sarcomeric length (RBM20: 1.747 ± 0.238 μm versus control: 1.404 ± 0.194 μm; P <0.0001) and decreased sarcomeric width (RBM20: 0.791 ± 0.609 μmversus control: 0.943 ± 0.166 μm; P <0.0001). Additionally, CMs showed defective Ca2+ handling machinery with prolonged Ca2+ levels in the cytoplasm as measured by greater area under the curve (RBM20: 814.718 ± 94.343 AU versus control: 206.941 ± 22.417 AU; P <0.05) and higher Ca2+ spike amplitude (RBM20: 35.281 ± 4.060 AU versus control:18.484 ± 1.518 AU; P <0.05). β-adrenergic stress induced with 10 μM norepinephrine demonstrated increased susceptibility to sarcomeric disorganization (RBM20: 86 ± 10.5% versus control: 40 ± 7%; P <0.001). This study features the first hiPSC model of RBM20 familial DCM. By monitoring human cardiac disease according to stage-specific cardiogenesis, this study demonstrates RBM20 familial DCM is a developmental disorder initiated by molecular defects that pattern maladaptive cellular mechanisms of pathological cardiac remodeling. Indeed, hiPSC-CMs recapitulate RBM20 familial DCM phenotype in a dish and establish a tool to dissect disease-relevant defects in RBM20 splicing as a global regulator of heart function.

Original languageEnglish (US)
Article numberddv468
Pages (from-to)254-265
Number of pages12
JournalHuman Molecular Genetics
Volume25
Issue number2
DOIs
StatePublished - Jan 15 2016

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Induced Pluripotent Stem Cells
RNA-Binding Proteins
Cardiac Myocytes
Dilated Cardiomyopathy
Missense Mutation
Familial dilated cardiomyopathy
RNA-Binding Motifs
Protein Splicing
Spliceosomes
Gene Expression Profiling
Adrenergic Agents
Genes
Area Under Curve
Heart Diseases
Norepinephrine

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Modeling structural and functional deficiencies of RBM20 familial dilated cardiomyopathy using human induced pluripotent stem cells. / Wyles, Saranya P.; Li, Xing; Hrstka, Sybil C.; Reyes, Santiago; Oommen, Saji; Beraldi, Rosanna; Edwards, Jessica; Terzic, Andre; Olson, Timothy Mark; Nelson, Timothy J.

In: Human Molecular Genetics, Vol. 25, No. 2, ddv468, 15.01.2016, p. 254-265.

Research output: Contribution to journalArticle

Wyles, Saranya P. ; Li, Xing ; Hrstka, Sybil C. ; Reyes, Santiago ; Oommen, Saji ; Beraldi, Rosanna ; Edwards, Jessica ; Terzic, Andre ; Olson, Timothy Mark ; Nelson, Timothy J. / Modeling structural and functional deficiencies of RBM20 familial dilated cardiomyopathy using human induced pluripotent stem cells. In: Human Molecular Genetics. 2016 ; Vol. 25, No. 2. pp. 254-265.
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