Mitotic phosphorylation stimulates DNA relaxation activity of human topoisomerase I

Jennifer S McDonald, Marina Galvez-Peralta, Nga T. Dai, David A. Loegering, Kevin L. Peterson, Xue W. Meng, Larry M Karnitz, Scott H Kaufmann

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Human DNA topoisomerase I (topo I) is an essential mammalian enzyme that regulates DNA supercoiling during transcription and replication. In addition, topo I is specifically targeted by the anticancer compound camptothecin and its derivatives. Previous studies have indicated that topo I is a phosphoprotein and that phosphorylation stimulates its DNA relaxation activity. The locations of most topo I phosphorylation sites have not been identified, preventing a more detailed examination of this modification. To address this issue, mass spectrometry was used to identify four topo I residues that are phosphorylated in intact cells: Ser10, Ser21, Ser112, and Ser394. Immunoblotting using anti-phosphoepitope antibodies demonstrated that these sites are phosphorylated during mitosis. In vitro kinase assays demonstrated that Ser10 can be phosphorylated by casein kinase II, Ser21 can be phosphorylated by protein kinase Cα, and Ser112 and Ser394 can be phosphorylated by Cdk1. When wild type topo I was pulled down from mitotic cells and dephosphorylated with alkaline phosphatase, topo I activity decreased 2-fold. Likewise, topo I polypeptide with all four phosphorylation sites mutated to alanine exhibited 2-fold lower DNA relaxation activity than wild type topo I after isolation from mitotic cells. Further mutational analysis demonstrated that Ser21 phosphorylation was responsible for this change. Consistent with these results, wild type topo I (but not S21A topo I) exhibited increased sensitivity to camptothecin-induced trapping onDNAduring mitosis. Collectively these results indicate that topo I is phosphorylated during mitosis at multiple sites, one of which enhances DNA relaxation activity in vitro and interaction with DNA in cells.

Original languageEnglish (US)
Pages (from-to)16711-16722
Number of pages12
JournalJournal of Biological Chemistry
Volume283
Issue number24
DOIs
StatePublished - Jun 13 2008

Fingerprint

Type I DNA Topoisomerase
Phosphorylation
Human Activities
DNA
Mitosis
Camptothecin
Casein Kinase II
Cell Separation
Phosphoproteins
Transcription
Immunoblotting
Alanine
Protein Kinase C
Mass spectrometry
Alkaline Phosphatase
Anti-Idiotypic Antibodies
Assays
Mass Spectrometry
Phosphotransferases

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Mitotic phosphorylation stimulates DNA relaxation activity of human topoisomerase I. / McDonald, Jennifer S; Galvez-Peralta, Marina; Dai, Nga T.; Loegering, David A.; Peterson, Kevin L.; Meng, Xue W.; Karnitz, Larry M; Kaufmann, Scott H.

In: Journal of Biological Chemistry, Vol. 283, No. 24, 13.06.2008, p. 16711-16722.

Research output: Contribution to journalArticle

McDonald, Jennifer S ; Galvez-Peralta, Marina ; Dai, Nga T. ; Loegering, David A. ; Peterson, Kevin L. ; Meng, Xue W. ; Karnitz, Larry M ; Kaufmann, Scott H. / Mitotic phosphorylation stimulates DNA relaxation activity of human topoisomerase I. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 24. pp. 16711-16722.
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