Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells

Sayyed K. Zaidi, Daniel W. Young, Shirwin M. Pockwinse, Amjad Javed, Jane B. Lian, Janet L. Stein, Andre J. Van Wijnen, Gary S. Stein

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately organized in the interphase nucleus and provide a model for understanding the subnuclear organization of tissue-specific regulatory proteins after mitosis. Here we have used quantitative in situ immunofluorescence microscopy and quantitative image analysis to show that Runx factors undergo progressive changes in cellular localization during mitosis while retaining a punctate distribution. In comparison, the acetyl transferase p300 and acetylated histone H4 remain localized with DNA throughout mitosis while the RNA processing factor SC35 is excluded from mitotic chromatin. Subnuclear organization of Runx foci is completely restored in telophase, and Runx proteins are equally partitioned into progeny nuclei. In contrast, subnuclear organization of SC35 is restored subsequent to telophase. Our results show a sequential reorganization of Runx and its coregulatory proteins that precedes restoration of RNA processing speckles. Thus, mitotic partitioning and spatiotemporal reorganization of regulatory proteins together render progeny cells equivalently competent to support phenotypic gene expression.

Original languageEnglish (US)
Pages (from-to)14852-14857
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number25
DOIs
StatePublished - Dec 9 2003

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells'. Together they form a unique fingerprint.

  • Cite this

    Zaidi, S. K., Young, D. W., Pockwinse, S. M., Javed, A., Lian, J. B., Stein, J. L., Van Wijnen, A. J., & Stein, G. S. (2003). Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells. Proceedings of the National Academy of Sciences of the United States of America, 100(25), 14852-14857. https://doi.org/10.1073/pnas.2533076100