Mitotic Inheritance of mRNA Facilitates Translational Activation of the Osteogenic-Lineage Commitment Factor Runx2 in Progeny of Osteoblastic Cells

Nelson Varela, Alejandra Aranguiz, Carlos Lizama, Hugo Sepulveda, Marcelo Antonelli, Roman Thaler, Ricardo D. Moreno, Martin Montecino, Gary S. Stein, Andre J van Wijnen, Mario Galindo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Epigenetic mechanisms mediate the acquisition of specialized cellular phenotypes during tissue development, maintenance and repair. When phenotype-committed cells transit through mitosis, chromosomal condensation counteracts epigenetic activation of gene expression. Subsequent post-mitotic re-activation of transcription depends on epigenetic DNA and histone modifications, as well as other architecturally bound proteins that "bookmark" the genome. Osteogenic lineage commitment, differentiation and progenitor proliferation require the bone-related runt-related transcription factor Runx2. Here, we characterized a non-genomic mRNA mediated mechanism by which osteoblast precursors retain their phenotype during self-renewal. We show that osteoblasts produce maximal levels of Runx2 mRNA, but not protein, prior to mitotic cell division. Runx2 mRNA partitions symmetrically between daughter cells in a non-chromosomal tubulin-containing compartment. Subsequently, transcription-independent de novo synthesis of Runx2 protein in early G1 phase results in increased functional interactions of Runx2 with a representative osteoblast-specific target gene (osteocalcin/BGLAP2) in chromatin. Somatic transmission of Runx2 mRNAs in osteoblasts and osteosarcoma cells represents a versatile mechanism for translational rather than transcriptional induction of this principal gene regulator to maintain osteoblast phenotype identity after mitosis.

Original languageEnglish (US)
Pages (from-to)1001-1014
Number of pages14
JournalJournal of Cellular Physiology
Volume231
Issue number5
DOIs
StatePublished - May 1 2016

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Osteoblasts
Chemical activation
Cells
Messenger RNA
Epigenomics
Phenotype
Genes
Transcription
Mitosis
Core Binding Factor Alpha 1 Subunit
Histone Code
Osteocalcin
G1 Phase
Osteosarcoma
Regulator Genes
Tubulin
Gene expression
Cell Division
Histones
Transcriptional Activation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Mitotic Inheritance of mRNA Facilitates Translational Activation of the Osteogenic-Lineage Commitment Factor Runx2 in Progeny of Osteoblastic Cells. / Varela, Nelson; Aranguiz, Alejandra; Lizama, Carlos; Sepulveda, Hugo; Antonelli, Marcelo; Thaler, Roman; Moreno, Ricardo D.; Montecino, Martin; Stein, Gary S.; van Wijnen, Andre J; Galindo, Mario.

In: Journal of Cellular Physiology, Vol. 231, No. 5, 01.05.2016, p. 1001-1014.

Research output: Contribution to journalArticle

Varela, N, Aranguiz, A, Lizama, C, Sepulveda, H, Antonelli, M, Thaler, R, Moreno, RD, Montecino, M, Stein, GS, van Wijnen, AJ & Galindo, M 2016, 'Mitotic Inheritance of mRNA Facilitates Translational Activation of the Osteogenic-Lineage Commitment Factor Runx2 in Progeny of Osteoblastic Cells', Journal of Cellular Physiology, vol. 231, no. 5, pp. 1001-1014. https://doi.org/10.1002/jcp.25188
Varela, Nelson ; Aranguiz, Alejandra ; Lizama, Carlos ; Sepulveda, Hugo ; Antonelli, Marcelo ; Thaler, Roman ; Moreno, Ricardo D. ; Montecino, Martin ; Stein, Gary S. ; van Wijnen, Andre J ; Galindo, Mario. / Mitotic Inheritance of mRNA Facilitates Translational Activation of the Osteogenic-Lineage Commitment Factor Runx2 in Progeny of Osteoblastic Cells. In: Journal of Cellular Physiology. 2016 ; Vol. 231, No. 5. pp. 1001-1014.
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