Mitochondrial metabolic function assessed in vivo and in vitro

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Purpose of review: Mitochondrial content and function vary across species, tissue types, and lifespan. Alterations in skeletal muscle mitochondrial function have been reported to occur in aging and in many other pathological conditions. This review focuses on the state of the art in-vivo and in-vitro methodologies for assessment of muscle mitochondrial function. Recent findings: Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental methods have recently been substantially improved and novel approaches to assess mitochondrial functions in vitro have emerged. Noninvasive methods based on magnetic resonance spectroscopy and near-infrared spectroscopy permit in-vivo assessment of mitochondrial function and are rapidly becoming more accessible to many investigators. Moreover, it is now possible to gather information on regulation of mitochondrial content by measuring the in-vivo synthesis rate of individual mitochondrial proteins. Summary: High-resolution respirometry has emerged as a powerful tool for in-vitro measurements of mitochondrial function in isolated mitochondria and permeabilized fibers. Direct measurements of adenosine triphosphate production are possible by bioluminescence. Mechanistic data provided by these methods is further complimented by in-vivo assessment using magnetic resonance spectroscopy and near-infrared spectroscopy and the translational rate of gene transcripts.

Original languageEnglish (US)
Pages (from-to)511-517
Number of pages7
JournalCurrent Opinion in Clinical Nutrition and Metabolic Care
Volume13
Issue number5
DOIs
StatePublished - Sep 2010

Fingerprint

Near-Infrared Spectroscopy
Mitochondria
Magnetic Resonance Spectroscopy
Mitochondrial Proteins
Skeletal Muscle
Adenosine Triphosphate
Research Personnel
Muscles
Genes
In Vitro Techniques

Keywords

  • magnetic resonance spectroscopy
  • mass spectrometry
  • mitochondria
  • respiration
  • skeletal muscle

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

@article{fc7ab8b77ee74b41a280be75333889be,
title = "Mitochondrial metabolic function assessed in vivo and in vitro",
abstract = "Purpose of review: Mitochondrial content and function vary across species, tissue types, and lifespan. Alterations in skeletal muscle mitochondrial function have been reported to occur in aging and in many other pathological conditions. This review focuses on the state of the art in-vivo and in-vitro methodologies for assessment of muscle mitochondrial function. Recent findings: Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental methods have recently been substantially improved and novel approaches to assess mitochondrial functions in vitro have emerged. Noninvasive methods based on magnetic resonance spectroscopy and near-infrared spectroscopy permit in-vivo assessment of mitochondrial function and are rapidly becoming more accessible to many investigators. Moreover, it is now possible to gather information on regulation of mitochondrial content by measuring the in-vivo synthesis rate of individual mitochondrial proteins. Summary: High-resolution respirometry has emerged as a powerful tool for in-vitro measurements of mitochondrial function in isolated mitochondria and permeabilized fibers. Direct measurements of adenosine triphosphate production are possible by bioluminescence. Mechanistic data provided by these methods is further complimented by in-vivo assessment using magnetic resonance spectroscopy and near-infrared spectroscopy and the translational rate of gene transcripts.",
keywords = "magnetic resonance spectroscopy, mass spectrometry, mitochondria, respiration, skeletal muscle",
author = "Lanza, {Ian R} and Nair, {K Sreekumaran}",
year = "2010",
month = "9",
doi = "10.1097/MCO.0b013e32833cc93d",
language = "English (US)",
volume = "13",
pages = "511--517",
journal = "Current Opinion in Clinical Nutrition and Metabolic Care",
issn = "1363-1950",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Mitochondrial metabolic function assessed in vivo and in vitro

AU - Lanza, Ian R

AU - Nair, K Sreekumaran

PY - 2010/9

Y1 - 2010/9

N2 - Purpose of review: Mitochondrial content and function vary across species, tissue types, and lifespan. Alterations in skeletal muscle mitochondrial function have been reported to occur in aging and in many other pathological conditions. This review focuses on the state of the art in-vivo and in-vitro methodologies for assessment of muscle mitochondrial function. Recent findings: Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental methods have recently been substantially improved and novel approaches to assess mitochondrial functions in vitro have emerged. Noninvasive methods based on magnetic resonance spectroscopy and near-infrared spectroscopy permit in-vivo assessment of mitochondrial function and are rapidly becoming more accessible to many investigators. Moreover, it is now possible to gather information on regulation of mitochondrial content by measuring the in-vivo synthesis rate of individual mitochondrial proteins. Summary: High-resolution respirometry has emerged as a powerful tool for in-vitro measurements of mitochondrial function in isolated mitochondria and permeabilized fibers. Direct measurements of adenosine triphosphate production are possible by bioluminescence. Mechanistic data provided by these methods is further complimented by in-vivo assessment using magnetic resonance spectroscopy and near-infrared spectroscopy and the translational rate of gene transcripts.

AB - Purpose of review: Mitochondrial content and function vary across species, tissue types, and lifespan. Alterations in skeletal muscle mitochondrial function have been reported to occur in aging and in many other pathological conditions. This review focuses on the state of the art in-vivo and in-vitro methodologies for assessment of muscle mitochondrial function. Recent findings: Classic studies of isolated mitochondria have measured function from maximal respiratory capacity. These fundamental methods have recently been substantially improved and novel approaches to assess mitochondrial functions in vitro have emerged. Noninvasive methods based on magnetic resonance spectroscopy and near-infrared spectroscopy permit in-vivo assessment of mitochondrial function and are rapidly becoming more accessible to many investigators. Moreover, it is now possible to gather information on regulation of mitochondrial content by measuring the in-vivo synthesis rate of individual mitochondrial proteins. Summary: High-resolution respirometry has emerged as a powerful tool for in-vitro measurements of mitochondrial function in isolated mitochondria and permeabilized fibers. Direct measurements of adenosine triphosphate production are possible by bioluminescence. Mechanistic data provided by these methods is further complimented by in-vivo assessment using magnetic resonance spectroscopy and near-infrared spectroscopy and the translational rate of gene transcripts.

KW - magnetic resonance spectroscopy

KW - mass spectrometry

KW - mitochondria

KW - respiration

KW - skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=77955919140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955919140&partnerID=8YFLogxK

U2 - 10.1097/MCO.0b013e32833cc93d

DO - 10.1097/MCO.0b013e32833cc93d

M3 - Article

C2 - 20616711

AN - SCOPUS:77955919140

VL - 13

SP - 511

EP - 517

JO - Current Opinion in Clinical Nutrition and Metabolic Care

JF - Current Opinion in Clinical Nutrition and Metabolic Care

SN - 1363-1950

IS - 5

ER -