Misregulated E-Cadherin expression associated with an aggressive brain tumor phenotype

Laura J. Lewis-Tuffin, Fausto Rodriguez, Caterina Giannini, Bernd Scheithauer, Brian M. Necela, Jann N Sarkaria, Panagiotis Z Anastasiadis

Research output: Contribution to journalArticle

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Abstract

Background: Cadherins are essential components of the adherens junction complexes that mediate cell-cell adhesion and regulate cell motility. During tissue morphogenesis, changes in cadherin expression (known as cadherin switching) are a common mechanism for altering cell fate. Cadherin switching is also common during epithelial tumor progression, where it is thought to promote tumor invasion and metastasis. E-cadherin is the predominant cadherin expressed in epithelial tissues, but its expression is very limited in normal brain. Methodology/Principal Findings:We identified E-cadherin expression in a retrospective series of glioblastomas exhibiting epithelial or pseudoepithelial differentiation. Unlike in epithelial tissues, E-cadherin expression in gliomas correlated with an unfavorable clinical outcome. Western blotting of two panels of human GBM cell lines propagated either as xenografts in nude mice or grown under conventional cell culture conditions confirmed that E-cadherin expression is rare. However, a small number of xenograft lines did express E-cadherin, its expression correlating with increased invasiveness when the cells were implanted orthotopically in mouse brain. In the conventionally cultured SF767 glioma cell line, E-cadherin expression was localized throughout the plasma membrane rather than being restricted to areas of cell-cell contact. SHRNA knockdown of E-cadherin in these cells resulted in decreased proliferation and migration in vitro. Conclusions/Significance: Our data shows an unexpected correlation between the abnormal expression of E-cadherin in a subset of GBM tumor cells and the growth and migration of this aggressive brain tumor subtype.

Original languageEnglish (US)
Article numbere13665
JournalPLoS One
Volume5
Issue number10
DOIs
StatePublished - 2010

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cadherins
Cadherins
Brain Neoplasms
Tumors
Brain
Phenotype
brain
phenotype
neoplasms
Cells
cells
Tissue
Heterografts
Glioma
Cell Movement
epithelium
Epithelium
Adherens Junctions
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Misregulated E-Cadherin expression associated with an aggressive brain tumor phenotype. / Lewis-Tuffin, Laura J.; Rodriguez, Fausto; Giannini, Caterina; Scheithauer, Bernd; Necela, Brian M.; Sarkaria, Jann N; Anastasiadis, Panagiotis Z.

In: PLoS One, Vol. 5, No. 10, e13665, 2010.

Research output: Contribution to journalArticle

Lewis-Tuffin, Laura J. ; Rodriguez, Fausto ; Giannini, Caterina ; Scheithauer, Bernd ; Necela, Brian M. ; Sarkaria, Jann N ; Anastasiadis, Panagiotis Z. / Misregulated E-Cadherin expression associated with an aggressive brain tumor phenotype. In: PLoS One. 2010 ; Vol. 5, No. 10.
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