Misdirection of regenerating motor axons after nerve injury and repair in the rat sciatic nerve model

Godard C W de Ruiter, Martijn J A Malessy, Awad O. Alaid, Robert J. Spinner, JaNean K. Engelstad, Eric James Sorenson, Kenton R Kaufman, Peter J Dyck, Anthony John Windebank

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Misdirection of regenerating axons is one of the factors that can explain the poor results often found after nerve injury and repair. In this study, we quantified the degree of misdirection and the effect on recovery of function after different types of nerve injury and repair in the rat sciatic nerve model; crush injury, direct coaptation, and autograft repair. Sequential tracing with retrograde labeling of the peroneal nerve before and 8 weeks after nerve injury and repair was performed to quantify the accuracy of motor axon regeneration. Digital video analysis of ankle motion was used to investigate the recovery of function. In addition, serial compound action potential recordings and nerve and muscle morphometry were performed. In our study, accuracy of motor axon regeneration was found to be limited; only 71% (± 4.9%) of the peroneal motoneurons were correctly directed 2 months after sciatic crush injury, 42% (± 4.2%) after direct coaptation, and 25% (± 6.6%) after autograft repair. Recovery of ankle motion was incomplete after all types of nerve injury and repair and demonstrated a disturbed balance of ankle plantar and dorsiflexion. The number of motoneurons from which axons had regenerated was not significantly different from normal. The number of myelinated axons was significantly increased distal to the site of injury. Misdirection of regenerating motor axons is a major factor in the poor recovery of nerves that innervate different muscles. The results of this study can be used as basis for developing new nerve repair techniques that may improve the accuracy of regeneration.

Original languageEnglish (US)
Pages (from-to)339-350
Number of pages12
JournalExperimental Neurology
Volume211
Issue number2
DOIs
StatePublished - Jun 2008

Fingerprint

Sciatic Nerve
Axons
Wounds and Injuries
Ankle
Regeneration
Recovery of Function
Autografts
Motor Neurons
Muscles
Peroneal Nerve
Action Potentials

Keywords

  • Aberrant reinnervation
  • Accuracy of regeneration
  • Ankle motion analysis
  • Double labeling
  • Sequential retrograde tracing

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Misdirection of regenerating motor axons after nerve injury and repair in the rat sciatic nerve model. / de Ruiter, Godard C W; Malessy, Martijn J A; Alaid, Awad O.; Spinner, Robert J.; Engelstad, JaNean K.; Sorenson, Eric James; Kaufman, Kenton R; Dyck, Peter J; Windebank, Anthony John.

In: Experimental Neurology, Vol. 211, No. 2, 06.2008, p. 339-350.

Research output: Contribution to journalArticle

de Ruiter, Godard C W ; Malessy, Martijn J A ; Alaid, Awad O. ; Spinner, Robert J. ; Engelstad, JaNean K. ; Sorenson, Eric James ; Kaufman, Kenton R ; Dyck, Peter J ; Windebank, Anthony John. / Misdirection of regenerating motor axons after nerve injury and repair in the rat sciatic nerve model. In: Experimental Neurology. 2008 ; Vol. 211, No. 2. pp. 339-350.
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