TY - JOUR
T1 - MiRNA expression in diffuse large B-cell lymphoma treated with chemoimmunotherapy
AU - Montes-Moreno, Santiago
AU - Martinez, Nerea
AU - Sanchez-Espiridión, Beatriz
AU - Uriarte, Ramon Díaz
AU - Rodriguez, Maria Elena
AU - Saez, Anabel
AU - Montalbán, Carlos
AU - Gomez, Gonzalo
AU - Pisano, David G.
AU - García, Juan Fernando
AU - Conde, Eulogio
AU - Gonzalez-Barca, Eva
AU - Lopez, Andres
AU - Mollejo, Manuela
AU - Grande, Carlos
AU - Martinez, Miguel Angel
AU - Dunphy, Cherie
AU - Hsi, Eric D.
AU - Rocque, Gabrielle B.
AU - Chang, Julie
AU - Go, Ronald S.
AU - Visco, Carlo
AU - Xu-Monette, Zijun
AU - Young, Ken H.
AU - Piris, Miguel A.
PY - 2011/7/28
Y1 - 2011/7/28
N2 - Diffuse large B-cell lymphoma (DLBCL) prognostication requires additional biologic markers. miRNAs may constitute markers for cancer diagnosis, outcome, or therapy response. In the present study, we analyzed the miRNA expression profile in a retrospective multicenter series of 258 DLBCL patients uniformly treated with chemoimmunotherapy. Findings were correlated with overall survival (OS) and progression-free survival (PFS). miRNAand gene-expression profiles were studied using microarrays in an initial set of 36 cases. A selection of miRNAs associated with either DLBCL molecular subtypes (GCB/ABC) or clinical outcome were studied by multiplex RT-PCR in a test group of 240 cases with available formalinfixed, paraffin-embedded (FFPE) diagnostic samples. The samples were divided into a training set (123 patients) and used to derive miRNA-based and combined (with IPI score) Cox regression models in an independent validation series (117 patients). Our model based on miRNA expression predicts OS and PFS and improves upon the predictions based on clinical variables. Combined models with IPI score identified a high-risk group of patients with a 2-yearOSand aPFSprobability of < 50%. In summary, a precise miRNA signature is associated with poor clinical outcome in chemoimmunotherapy-treated DLBCL patients. This information improves upon IPI-based predictions and identifies a subgroup of candidate patients for alternative therapeutic regimens.
AB - Diffuse large B-cell lymphoma (DLBCL) prognostication requires additional biologic markers. miRNAs may constitute markers for cancer diagnosis, outcome, or therapy response. In the present study, we analyzed the miRNA expression profile in a retrospective multicenter series of 258 DLBCL patients uniformly treated with chemoimmunotherapy. Findings were correlated with overall survival (OS) and progression-free survival (PFS). miRNAand gene-expression profiles were studied using microarrays in an initial set of 36 cases. A selection of miRNAs associated with either DLBCL molecular subtypes (GCB/ABC) or clinical outcome were studied by multiplex RT-PCR in a test group of 240 cases with available formalinfixed, paraffin-embedded (FFPE) diagnostic samples. The samples were divided into a training set (123 patients) and used to derive miRNA-based and combined (with IPI score) Cox regression models in an independent validation series (117 patients). Our model based on miRNA expression predicts OS and PFS and improves upon the predictions based on clinical variables. Combined models with IPI score identified a high-risk group of patients with a 2-yearOSand aPFSprobability of < 50%. In summary, a precise miRNA signature is associated with poor clinical outcome in chemoimmunotherapy-treated DLBCL patients. This information improves upon IPI-based predictions and identifies a subgroup of candidate patients for alternative therapeutic regimens.
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UR - http://www.scopus.com/inward/citedby.url?scp=79961017480&partnerID=8YFLogxK
U2 - 10.1182/blood-2010-11-321554
DO - 10.1182/blood-2010-11-321554
M3 - Article
C2 - 21633089
AN - SCOPUS:79961017480
SN - 0006-4971
VL - 118
SP - 1034
EP - 1040
JO - Blood
JF - Blood
IS - 4
ER -