Minor pocket B influences peptide binding, peptide presentation and alloantigenicity of H-2Kb

Theodore J. Yun, Michelle D. Tallquist, Eric M. Rohren, James M. Shell, Larry R Pease

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microsequence analysis of peptides eluted from the murine class I H-2Kb molecule together with the three-dimensional structure of the molecule co-crystallized with a homogenous population of peptides suggests that pocket B is a minor pocket that does not play a major role in peptide presentation. This is in contrast to most other class I molecules in which pocket B plays a central role in selecting and presenting antigenic peptides. To investigate the role of pocket B in antigen presentation by the Kb molecule, we analyzed site-directed mutants of position 45 in pocket B for their effect on both allo- and peptide-specific recognition. We made an identical set of mutations in Kbm8 at residue 45 in order to evaluate their influence in the context of a more open pocket B which results from the bm8 substitution at amino acid 24 (E → S). We demonstrated that this minor pocket did play a significant role in the antigenicity of both molecules and that this role was more readily apparent in the context of the more open pocket B of Kbm8. In addition, we found that some substitutions of residue 45 in the Kbms molecule restored recognition by some alloreactive and peptide specific anti-Kb T cell clones which are normally restricted to Kb, indicating that multiple configurations of amino acids in a pocket could result in similar binding and presentation capabilities.

Original languageEnglish (US)
Pages (from-to)1037-1047
Number of pages11
JournalInternational Immunology
Volume6
Issue number7
DOIs
StatePublished - Jul 1994

Fingerprint

Peptides
Minor
Molecules
Substitution
Amino Acids
Amino acids
Substitution reactions
Antigen Presentation
T-cells
Amino Acid Substitution
Antigens
Clone
Mutant
Presentation
Influence
peptide B
Mutation
Clone Cells
T-Lymphocytes
Three-dimensional

Keywords

  • Class I
  • MHC
  • Ovalbumin
  • Site-directed mutagenesis
  • T cell epitope

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation
  • Immunology

Cite this

Minor pocket B influences peptide binding, peptide presentation and alloantigenicity of H-2Kb. / Yun, Theodore J.; Tallquist, Michelle D.; Rohren, Eric M.; Shell, James M.; Pease, Larry R.

In: International Immunology, Vol. 6, No. 7, 07.1994, p. 1037-1047.

Research output: Contribution to journalArticle

Yun, Theodore J. ; Tallquist, Michelle D. ; Rohren, Eric M. ; Shell, James M. ; Pease, Larry R. / Minor pocket B influences peptide binding, peptide presentation and alloantigenicity of H-2Kb. In: International Immunology. 1994 ; Vol. 6, No. 7. pp. 1037-1047.
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