Minimally invasive monitoring of CD4 T cells at multiple mucosal tissues after intranasal vaccination in rhesus macaques

Stephanie Dorta-Estremera, Pramod N. Nehete, Guojun Yang, Hong He, Bharti P. Nehete, Kathryn K. Shelton, Michael A Barry, K. Jagannadha Sastry

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Studies in nonhuman primates (NHP) for prospective immune cell monitoring subsequent to infection and/or vaccination usually rely on periodic sampling of the blood samples with only occasional collections of biopsies from mucosal tissues because of safety concerns and practical constraints. Here we present evidence in support of cytobrush sampling of oral, rectal, and genital mucosal tissues as a minimally invasive approach for the phenotypic analyses of different T cells subsets de novo as well as prospectively after intranasal immunization in rhesus macaques. Significant percentages of viable lymphocytes were obtained consistently from both naïve and chronically SIV-infected rhesus macaques. The percentages of CD3+ T cells in the blood were significantly higher compared to those in the mucosal tissues analyzed in the naïve animals, while in the SIV+ animals the CD3+ T cells were significantly elevated in the rectal tissues, relative to all other sites analyzed. In the naïve, but not SIV+ macaques, the rectal and vaginal mucosal tissues, compared to oral mucosa and blood, showed higher diversity and percentages of CD4+ T cells expressing the HIV entry co-receptor CCR5 and mucosal specific adhesion (CD103) as well as activation (HLA-DR) and proliferation (Ki67) markers. Sequential daily cytobrush sampling from the oral, rectal, and genital mucosal tissues was performed in SIV+ animals from an ongoing study where they were administered intranasal immunization with adenoviral vectored vaccines incorporating the green fluorescent protein (GFP) reporter gene. We detected a transient increase in GFP+ CD4 T cells in only oral mucosa suggesting limited mucosal trafficking. In general, CD4+ and CD8+ T cells expressing Ki67 transiently increased in all mucosal tissues, but those expressing the CCR5, HLA-DR, and CD103 markers exhibited minor changes. We propose the minimally invasive cytobrush sampling as a practical approach for effective and prospective immune monitoring of the oral-genital mucosal tissues in NHP.

Original languageEnglish (US)
Article numbere0188807
JournalPLoS One
Volume12
Issue number12
DOIs
StatePublished - Dec 1 2017

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T-cells
Macaca mulatta
Mucous Membrane
Vaccination
T-lymphocytes
vaccination
T-Lymphocytes
Monitoring
monitoring
digestive tract mucosa
Immunologic Monitoring
genitalia
mouth
Mouth Mucosa
HLA-DR Antigens
Sampling
Immunization
Green Fluorescent Proteins
Animals
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dorta-Estremera, S., Nehete, P. N., Yang, G., He, H., Nehete, B. P., Shelton, K. K., ... Sastry, K. J. (2017). Minimally invasive monitoring of CD4 T cells at multiple mucosal tissues after intranasal vaccination in rhesus macaques. PLoS One, 12(12), [e0188807]. https://doi.org/10.1371/journal.pone.0188807

Minimally invasive monitoring of CD4 T cells at multiple mucosal tissues after intranasal vaccination in rhesus macaques. / Dorta-Estremera, Stephanie; Nehete, Pramod N.; Yang, Guojun; He, Hong; Nehete, Bharti P.; Shelton, Kathryn K.; Barry, Michael A; Sastry, K. Jagannadha.

In: PLoS One, Vol. 12, No. 12, e0188807, 01.12.2017.

Research output: Contribution to journalArticle

Dorta-Estremera, S, Nehete, PN, Yang, G, He, H, Nehete, BP, Shelton, KK, Barry, MA & Sastry, KJ 2017, 'Minimally invasive monitoring of CD4 T cells at multiple mucosal tissues after intranasal vaccination in rhesus macaques', PLoS One, vol. 12, no. 12, e0188807. https://doi.org/10.1371/journal.pone.0188807
Dorta-Estremera, Stephanie ; Nehete, Pramod N. ; Yang, Guojun ; He, Hong ; Nehete, Bharti P. ; Shelton, Kathryn K. ; Barry, Michael A ; Sastry, K. Jagannadha. / Minimally invasive monitoring of CD4 T cells at multiple mucosal tissues after intranasal vaccination in rhesus macaques. In: PLoS One. 2017 ; Vol. 12, No. 12.
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