Miniaturized Multichannel Near Infrared Endoscope for Mouse Imaging

Martin A. Funovics, Herlen Alencar, Henry S. Su, Khashayarsha Khazaie, Ralph Weissleder, Umar Mahmood

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

We describe the design and construction of a miniaturized multichannel near infrared (NIR) endoscopic imaging system developed for high-resolution imaging of mice. The device allows for simultaneous real-time video images in white light and two independent NIR channels. Testing demonstrated independent acquisition of nanomolar concentrations of fluorochromes Cy5.5 and Cy7. Cross-talk between the NIR channels, partially a result of broad tails in the spectra of commonly used organic fluorochromes, was assessed, modeled for the linear range of the concentration/signal intensity function, and compensated. The calculated compensation was 5.5% and 22% of the total signal intensity in the two channels NIR 700 and NIR 780, respectively, at equal concentrations of the two fluorochromes. Using a mouse model of colonic adenomatosis, we show that both perfusion and protease activity can be detected simultaneously, independently, and repeatedly in live mice. The developed device should be useful for in vivo imaging of diverse molecular targets.

Original languageEnglish (US)
Pages (from-to)350-357
Number of pages8
JournalMolecular Imaging
Volume2
Issue number4
DOIs
StatePublished - Oct 1 2003

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

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    Funovics, M. A., Alencar, H., Su, H. S., Khazaie, K., Weissleder, R., & Mahmood, U. (2003). Miniaturized Multichannel Near Infrared Endoscope for Mouse Imaging. Molecular Imaging, 2(4), 350-357. https://doi.org/10.1162/153535003322750691