Mini-osmotic pump infusion model to investigate the systemic effects of chronic continuous exposure to staphylococcal superantigen in mice

Ashton L. Krogman, Vaidehi Chowdhary, Govindarajan Rajagopalan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Staphylococcus aureus can exist as a colonizer or can cause a spectrum of diseases. S. aureus elaborates several exotoxins and the superantigens are one among them. Staphylococcal superantigens (SSAg) cause robust activation of the immune system and acute exposure to significant amounts of SSAg can be potentially lethal. However, chronic exposure to SSAg is also possible. Administering SSAg using mini-osmotic pumps may mimic chronic recurrent exposure to SSAg. This is a relatively simple and safe way to administer purified SSAg or any other toxin/agent. In this chapter, we describe the mini-osmotic pump-mediated delivery of SSAg.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages109-114
Number of pages6
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1396
ISSN (Print)1064-3745

Keywords

  • Mini-osmotic pump
  • Staphylococcus aureus
  • Superantigen

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Krogman, A. L., Chowdhary, V., & Rajagopalan, G. (2016). Mini-osmotic pump infusion model to investigate the systemic effects of chronic continuous exposure to staphylococcal superantigen in mice. In Methods in Molecular Biology (pp. 109-114). (Methods in Molecular Biology; Vol. 1396). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3344-0_9