Microvascular function in younger adults with obesity and metabolic syndrome: Role of oxidative stress

Jacqueline K. Limberg, John W. Harrell, Rebecca E. Johansson, Marlowe W. Eldridge, Lester T. Proctor, Joshua J. Sebranek, William G. Schrage

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Older adults with cardiovascular disease exhibit microvascular dysfunction and increased levels of reactive oxygen species (ROS). We hypothesized that microvascular impairments begin early in the disease process and can be improved by scavenging ROS. Forearm blood flow (Doppler ultrasound) was measured in 45 young (32 ± 2 yr old) adults (n = 15/group) classified as lean, obese, and metabolic syndrome (MetSyn). Vasodilation in response to endothelial (ACh) and vascular smooth muscle [nitroprusside (NTP) and epoprostenol (Epo)] agonists was tested before and after intra-arterial infusion of ascorbic acid to scavenge ROS. Vasodilation was assessed as a rise in relative vascular conductance (ml·min -1·dl -1·100 mmHg -1). ACh and NTP responses were preserved (P = 0.825 and P = 0.924, respectively), whereas Epo responses were lower in obese and MetSyn adults (P<0.05) than in lean controls. Scavenging of ROS via infusion of ascorbic acid resulted in an increase in ACh-mediated (P < 0.001) and NTP-mediated (P<0.001) relative vascular conductance across all groups, suggesting that oxidative stress influences vascular responsiveness in adults with and without overt cardiovascular disease risk. Ascorbic acid had no effect on Epo-mediated vasodilation (P = 0.267). These results suggest that obese and MetSyn adults exhibit preserved endothelium-dependent vasodilation with reduced dependence on prostacyclin and are consistent with an upregulation of compensatory vascular control mechanisms.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume305
Issue number8
DOIs
StatePublished - Oct 15 2013
Externally publishedYes

Fingerprint

Epoprostenol
Vasodilation
Blood Vessels
Young Adult
Reactive Oxygen Species
Nitroprusside
Oxidative Stress
Obesity
Ascorbic Acid
Cardiovascular Diseases
Doppler Ultrasonography
Intra Arterial Infusions
Vascular Smooth Muscle
Forearm
Endothelium
Up-Regulation

Keywords

  • Blood flow
  • Endothelial function
  • Nitric oxide
  • Prediabetes
  • Prostacyclin

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Microvascular function in younger adults with obesity and metabolic syndrome : Role of oxidative stress. / Limberg, Jacqueline K.; Harrell, John W.; Johansson, Rebecca E.; Eldridge, Marlowe W.; Proctor, Lester T.; Sebranek, Joshua J.; Schrage, William G.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 305, No. 8, 15.10.2013.

Research output: Contribution to journalArticle

Limberg, Jacqueline K. ; Harrell, John W. ; Johansson, Rebecca E. ; Eldridge, Marlowe W. ; Proctor, Lester T. ; Sebranek, Joshua J. ; Schrage, William G. / Microvascular function in younger adults with obesity and metabolic syndrome : Role of oxidative stress. In: American Journal of Physiology - Heart and Circulatory Physiology. 2013 ; Vol. 305, No. 8.
@article{d99d7976c8304d7c945ce0f8bd1acb56,
title = "Microvascular function in younger adults with obesity and metabolic syndrome: Role of oxidative stress",
abstract = "Older adults with cardiovascular disease exhibit microvascular dysfunction and increased levels of reactive oxygen species (ROS). We hypothesized that microvascular impairments begin early in the disease process and can be improved by scavenging ROS. Forearm blood flow (Doppler ultrasound) was measured in 45 young (32 ± 2 yr old) adults (n = 15/group) classified as lean, obese, and metabolic syndrome (MetSyn). Vasodilation in response to endothelial (ACh) and vascular smooth muscle [nitroprusside (NTP) and epoprostenol (Epo)] agonists was tested before and after intra-arterial infusion of ascorbic acid to scavenge ROS. Vasodilation was assessed as a rise in relative vascular conductance (ml·min -1·dl -1·100 mmHg -1). ACh and NTP responses were preserved (P = 0.825 and P = 0.924, respectively), whereas Epo responses were lower in obese and MetSyn adults (P<0.05) than in lean controls. Scavenging of ROS via infusion of ascorbic acid resulted in an increase in ACh-mediated (P < 0.001) and NTP-mediated (P<0.001) relative vascular conductance across all groups, suggesting that oxidative stress influences vascular responsiveness in adults with and without overt cardiovascular disease risk. Ascorbic acid had no effect on Epo-mediated vasodilation (P = 0.267). These results suggest that obese and MetSyn adults exhibit preserved endothelium-dependent vasodilation with reduced dependence on prostacyclin and are consistent with an upregulation of compensatory vascular control mechanisms.",
keywords = "Blood flow, Endothelial function, Nitric oxide, Prediabetes, Prostacyclin",
author = "Limberg, {Jacqueline K.} and Harrell, {John W.} and Johansson, {Rebecca E.} and Eldridge, {Marlowe W.} and Proctor, {Lester T.} and Sebranek, {Joshua J.} and Schrage, {William G.}",
year = "2013",
month = "10",
day = "15",
doi = "10.1152/ajpheart.00291.2013",
language = "English (US)",
volume = "305",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "8",

}

TY - JOUR

T1 - Microvascular function in younger adults with obesity and metabolic syndrome

T2 - Role of oxidative stress

AU - Limberg, Jacqueline K.

AU - Harrell, John W.

AU - Johansson, Rebecca E.

AU - Eldridge, Marlowe W.

AU - Proctor, Lester T.

AU - Sebranek, Joshua J.

AU - Schrage, William G.

PY - 2013/10/15

Y1 - 2013/10/15

N2 - Older adults with cardiovascular disease exhibit microvascular dysfunction and increased levels of reactive oxygen species (ROS). We hypothesized that microvascular impairments begin early in the disease process and can be improved by scavenging ROS. Forearm blood flow (Doppler ultrasound) was measured in 45 young (32 ± 2 yr old) adults (n = 15/group) classified as lean, obese, and metabolic syndrome (MetSyn). Vasodilation in response to endothelial (ACh) and vascular smooth muscle [nitroprusside (NTP) and epoprostenol (Epo)] agonists was tested before and after intra-arterial infusion of ascorbic acid to scavenge ROS. Vasodilation was assessed as a rise in relative vascular conductance (ml·min -1·dl -1·100 mmHg -1). ACh and NTP responses were preserved (P = 0.825 and P = 0.924, respectively), whereas Epo responses were lower in obese and MetSyn adults (P<0.05) than in lean controls. Scavenging of ROS via infusion of ascorbic acid resulted in an increase in ACh-mediated (P < 0.001) and NTP-mediated (P<0.001) relative vascular conductance across all groups, suggesting that oxidative stress influences vascular responsiveness in adults with and without overt cardiovascular disease risk. Ascorbic acid had no effect on Epo-mediated vasodilation (P = 0.267). These results suggest that obese and MetSyn adults exhibit preserved endothelium-dependent vasodilation with reduced dependence on prostacyclin and are consistent with an upregulation of compensatory vascular control mechanisms.

AB - Older adults with cardiovascular disease exhibit microvascular dysfunction and increased levels of reactive oxygen species (ROS). We hypothesized that microvascular impairments begin early in the disease process and can be improved by scavenging ROS. Forearm blood flow (Doppler ultrasound) was measured in 45 young (32 ± 2 yr old) adults (n = 15/group) classified as lean, obese, and metabolic syndrome (MetSyn). Vasodilation in response to endothelial (ACh) and vascular smooth muscle [nitroprusside (NTP) and epoprostenol (Epo)] agonists was tested before and after intra-arterial infusion of ascorbic acid to scavenge ROS. Vasodilation was assessed as a rise in relative vascular conductance (ml·min -1·dl -1·100 mmHg -1). ACh and NTP responses were preserved (P = 0.825 and P = 0.924, respectively), whereas Epo responses were lower in obese and MetSyn adults (P<0.05) than in lean controls. Scavenging of ROS via infusion of ascorbic acid resulted in an increase in ACh-mediated (P < 0.001) and NTP-mediated (P<0.001) relative vascular conductance across all groups, suggesting that oxidative stress influences vascular responsiveness in adults with and without overt cardiovascular disease risk. Ascorbic acid had no effect on Epo-mediated vasodilation (P = 0.267). These results suggest that obese and MetSyn adults exhibit preserved endothelium-dependent vasodilation with reduced dependence on prostacyclin and are consistent with an upregulation of compensatory vascular control mechanisms.

KW - Blood flow

KW - Endothelial function

KW - Nitric oxide

KW - Prediabetes

KW - Prostacyclin

UR - http://www.scopus.com/inward/record.url?scp=84885608137&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885608137&partnerID=8YFLogxK

U2 - 10.1152/ajpheart.00291.2013

DO - 10.1152/ajpheart.00291.2013

M3 - Article

C2 - 23934859

AN - SCOPUS:84885608137

VL - 305

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 8

ER -