Microtubule nucleating capacity of centrosomes in tissue sections

Jeffrey L. Salisbury, Wilma L. Lingle, Richard A. White, Lynn E.M. Cordes, Susan Barrett

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We used a novel adaptation of methods for microtubule polymerization in vitro to assess the MTOC activity of centrosomes in frozen-sectioned tissues. Remarkably, centrosomes of tissue sections retain the ability to nucleate microtubules even after several years of storage as frozen tissue blocks. Adaptations of these methods allow accurate counts of microtubules from individual cells and the quantitative estimation the MTOC activity of the intact tissue. These methods can be utilized to characterize MTOC activity in normal and diseased tissues and in particular tissues at different stages of development.

Original languageEnglish (US)
Pages (from-to)1265-1273
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume47
Issue number10
DOIs
StatePublished - Oct 1999

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Keywords

  • Cytoskeleton
  • Development
  • Disease
  • MTOC
  • Polymer

ASJC Scopus subject areas

  • Anatomy
  • Histology

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