Microtubule associated tumor suppressor 1 interacts with mitofusins to regulate mitochondrial morphology in endothelial cells

Yinfang Wang, Yitong Huang, Youbin Liu, Jinping Li, Yilong Hao, Peihao Yin, Zongjun Liu, Jingzhou Chen, Ying Wang, Nanping Wang, Peng Zhang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Mitochondria are dynamic organelles that are able to change their morphology and cellular distribution by either fission or fusion. However, the molecular mechanisms controlling mitochondrial dynamics in vascular endothelial cells (ECs) remain largely unknown. In this study, we observed that knockdown of microtubule-associated tumor suppressor 1 (MTUS1) in ECs inhibited tube formation and migration, accompanied with decreased promigratory signalings. We showed that MTUS1 was localized in the outer membrane of mitochondria in ECs. Knockdown of MTUS1 disturbed the elongated mitochondrial network and induced the formation of perinuclear clusters of mitochondria. Importantly, mitochondrial motility and fusion were suppressed, whereas generation of reactive oxygen species was increased in MTUS1 knockdown ECs. Mechanistically, we showed that the N-terminal coiled-coil domain of MTUS1 interacted with the mitochondrial membrane proteins, mitofusin-1 and mitofusin-2, to maintain mitochondrial morphology in ECs. This study illustrated a novel role of MTUS1 in mitochondrial morphology and EC angiogenic responses.

Original languageEnglish (US)
Pages (from-to)4504-4518
Number of pages15
JournalFASEB Journal
Volume32
Issue number8
DOIs
StatePublished - Aug 2018

Keywords

  • Angiogenesis
  • MTUS1
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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