Microsatellite instability in adenocarcinoma of the prostate

R. B. Terrell, A. H. Wille, J. C. Cheville, A. M. Nystuen, M. B. Cohen, V. C. Sheffield

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Instability of dinucleotide tandem repeat sequences has been reported to play a major role in the carcinogenic pathway of familial colon cancer, as well as a potential role in the carcinogenesis of other sporadic neoplasms. To determine the frequency of short tandem repeat instability in adenocarcinoma of the prostate, we studied 40 tumors that were stratified according to tumor grade. The tissue samples were screened with di-, tri- and tetranucelotide markers spanning a wide range of chromosomal loci, including an androgen receptor gene trinucleotide repeat. Microsatellite instability was observed overall in only one of the 40 (2.5%) prostate adenocarcinomas studied. This replication error-positive tumor demonstrated repeat length alterations at two loci. Five other tumors showed an alteration in microsatellite size at a single locus. These tumors were not considered to have the microsatellite instability phenotype. All changes were identified either within tetranucleotide sequences or within tetranucleotide sequences or within the androgen receptor gene repeat (4 of 20 total markers analyzed). Both repeat length expansions and contractions were identified. The replication error-positive case also included separate metastatic nodal tissue. Additional microsatellite analysis of the metastatic tumor tissue revealed allelic patterns identical with the normal tissue control. Our data indicate that microsatellite instability is rare in prostate adenocarcinoma. Therefore, observation of this low replication error frequency suggests that most prostate carcinomas develop in the absence of widespread accumulation of somatic mutations in short tandem repeat sequences. Additionally, these genetic alterations appear to occur more often in tetranucleotide repeat sequences as well as in an androgen receptor gene trinucleotide repeat.

Original languageEnglish (US)
Pages (from-to)799-805
Number of pages7
JournalAmerican Journal of Pathology
Volume147
Issue number3
StatePublished - 1995
Externally publishedYes

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Microsatellite Instability
Prostate
Adenocarcinoma
Microsatellite Repeats
Androgen Receptors
Neoplasms
Trinucleotide Repeats
Tandem Repeat Sequences
Genes
Dinucleotide Repeats
Colonic Neoplasms
Carcinogenesis
Observation
Carcinoma
Phenotype

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Terrell, R. B., Wille, A. H., Cheville, J. C., Nystuen, A. M., Cohen, M. B., & Sheffield, V. C. (1995). Microsatellite instability in adenocarcinoma of the prostate. American Journal of Pathology, 147(3), 799-805.

Microsatellite instability in adenocarcinoma of the prostate. / Terrell, R. B.; Wille, A. H.; Cheville, J. C.; Nystuen, A. M.; Cohen, M. B.; Sheffield, V. C.

In: American Journal of Pathology, Vol. 147, No. 3, 1995, p. 799-805.

Research output: Contribution to journalArticle

Terrell, RB, Wille, AH, Cheville, JC, Nystuen, AM, Cohen, MB & Sheffield, VC 1995, 'Microsatellite instability in adenocarcinoma of the prostate', American Journal of Pathology, vol. 147, no. 3, pp. 799-805.
Terrell RB, Wille AH, Cheville JC, Nystuen AM, Cohen MB, Sheffield VC. Microsatellite instability in adenocarcinoma of the prostate. American Journal of Pathology. 1995;147(3):799-805.
Terrell, R. B. ; Wille, A. H. ; Cheville, J. C. ; Nystuen, A. M. ; Cohen, M. B. ; Sheffield, V. C. / Microsatellite instability in adenocarcinoma of the prostate. In: American Journal of Pathology. 1995 ; Vol. 147, No. 3. pp. 799-805.
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