Microsatellite instability at AAAG repeat sequences in respiratory tract cancers

Lihua Xu, John Chow, Julie Bonacum, Claus Eisenberger, Steve A. Ahrendt, Michael Spafford, Li Wu, Sheng M. Lee, Steven Piantadosi, Melvyn S. Tockman, David Sidransky, Jin Jen

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

We surveyed the occurrence of novel alleles at microsatellite sequences in non-small cell lung cancers (NSCLC) using 61 tetranucleotide repeat markers. The presence of at least one new allele, consistent with microsatellite instability (MSI), was observed in 26 of 61 (43%) markers involving 30 of 47 (64%) NSCLC. Twelve of the 26 markers detected new alleles in 2 or more tumors and 11 of these 12 markers contained an AAAG repeat sequence. Using this panel of 12 markers, MSI was detected in 24 of 47 (51%) NSCLC and 10 of 18 (56%) head and neck cancers but was only observed in 8 of 38 (21%) bladder cancers and 3 of 25 (12%) kidney cancers. Our results suggested that about 50% of respiratory tract cancers exhibited microsatellite instability predominantly at AAAG sequences. This distinct type of instability was termed EMAST for elevated microsatellite alterations at selected tetranucleotide repeats. The identification of markers with EMAST should have potential application for the molecular detection of respiratory tract cancers.

Original languageEnglish (US)
Pages (from-to)200-204
Number of pages5
JournalInternational Journal of Cancer
Volume91
Issue number2
DOIs
StatePublished - Jan 15 2001

Keywords

  • AAAG repeats
  • Cancer genetics
  • Microsatellite instability
  • NSCLC
  • Tumor detection

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Xu, L., Chow, J., Bonacum, J., Eisenberger, C., Ahrendt, S. A., Spafford, M., Wu, L., Lee, S. M., Piantadosi, S., Tockman, M. S., Sidransky, D., & Jen, J. (2001). Microsatellite instability at AAAG repeat sequences in respiratory tract cancers. International Journal of Cancer, 91(2), 200-204. https://doi.org/10.1002/1097-0215(200002)9999:9999<::AID-IJC1031>3.0.CO;2-0