Microsatellite instability at AAAG repeat sequences in respiratory tract cancers

Lihua Xu, John Chow, Julie Bonacum, Claus Eisenberger, Steve A. Ahrendt, Michael Spafford, Li Wu, Sheng M. Lee, Steven Piantadosi, Melvyn S. Tockman, David Sidransky, Jin Jen

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We surveyed the occurrence of novel alleles at microsatellite sequences in non-small cell lung cancers (NSCLC) using 61 tetranucleotide repeat markers. The presence of at least one new allele, consistent with microsatellite instability (MSI), was observed in 26 of 61 (43%) markers involving 30 of 47 (64%) NSCLC. Twelve of the 26 markers detected new alleles in 2 or more tumors and 11 of these 12 markers contained an AAAG repeat sequence. Using this panel of 12 markers, MSI was detected in 24 of 47 (51%) NSCLC and 10 of 18 (56%) head and neck cancers but was only observed in 8 of 38 (21%) bladder cancers and 3 of 25 (12%) kidney cancers. Our results suggested that about 50% of respiratory tract cancers exhibited microsatellite instability predominantly at AAAG sequences. This distinct type of instability was termed EMAST for elevated microsatellite alterations at selected tetranucleotide repeats. The identification of markers with EMAST should have potential application for the molecular detection of respiratory tract cancers.

Original languageEnglish (US)
Pages (from-to)200-204
Number of pages5
JournalInternational Journal of Cancer
Volume91
Issue number2
DOIs
StatePublished - Jan 15 2001
Externally publishedYes

Fingerprint

Microsatellite Instability
Respiratory System
Microsatellite Repeats
Non-Small Cell Lung Carcinoma
Alleles
Neoplasms
Kidney Neoplasms
Head and Neck Neoplasms
Urinary Bladder Neoplasms

Keywords

  • AAAG repeats
  • Cancer genetics
  • Microsatellite instability
  • NSCLC
  • Tumor detection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Microsatellite instability at AAAG repeat sequences in respiratory tract cancers. / Xu, Lihua; Chow, John; Bonacum, Julie; Eisenberger, Claus; Ahrendt, Steve A.; Spafford, Michael; Wu, Li; Lee, Sheng M.; Piantadosi, Steven; Tockman, Melvyn S.; Sidransky, David; Jen, Jin.

In: International Journal of Cancer, Vol. 91, No. 2, 15.01.2001, p. 200-204.

Research output: Contribution to journalArticle

Xu, L, Chow, J, Bonacum, J, Eisenberger, C, Ahrendt, SA, Spafford, M, Wu, L, Lee, SM, Piantadosi, S, Tockman, MS, Sidransky, D & Jen, J 2001, 'Microsatellite instability at AAAG repeat sequences in respiratory tract cancers', International Journal of Cancer, vol. 91, no. 2, pp. 200-204. https://doi.org/10.1002/1097-0215(200002)9999:9999<::AID-IJC1031>3.0.CO;2-0
Xu, Lihua ; Chow, John ; Bonacum, Julie ; Eisenberger, Claus ; Ahrendt, Steve A. ; Spafford, Michael ; Wu, Li ; Lee, Sheng M. ; Piantadosi, Steven ; Tockman, Melvyn S. ; Sidransky, David ; Jen, Jin. / Microsatellite instability at AAAG repeat sequences in respiratory tract cancers. In: International Journal of Cancer. 2001 ; Vol. 91, No. 2. pp. 200-204.
@article{8c95821c3810409cba50d78a9d2bf762,
title = "Microsatellite instability at AAAG repeat sequences in respiratory tract cancers",
abstract = "We surveyed the occurrence of novel alleles at microsatellite sequences in non-small cell lung cancers (NSCLC) using 61 tetranucleotide repeat markers. The presence of at least one new allele, consistent with microsatellite instability (MSI), was observed in 26 of 61 (43{\%}) markers involving 30 of 47 (64{\%}) NSCLC. Twelve of the 26 markers detected new alleles in 2 or more tumors and 11 of these 12 markers contained an AAAG repeat sequence. Using this panel of 12 markers, MSI was detected in 24 of 47 (51{\%}) NSCLC and 10 of 18 (56{\%}) head and neck cancers but was only observed in 8 of 38 (21{\%}) bladder cancers and 3 of 25 (12{\%}) kidney cancers. Our results suggested that about 50{\%} of respiratory tract cancers exhibited microsatellite instability predominantly at AAAG sequences. This distinct type of instability was termed EMAST for elevated microsatellite alterations at selected tetranucleotide repeats. The identification of markers with EMAST should have potential application for the molecular detection of respiratory tract cancers.",
keywords = "AAAG repeats, Cancer genetics, Microsatellite instability, NSCLC, Tumor detection",
author = "Lihua Xu and John Chow and Julie Bonacum and Claus Eisenberger and Ahrendt, {Steve A.} and Michael Spafford and Li Wu and Lee, {Sheng M.} and Steven Piantadosi and Tockman, {Melvyn S.} and David Sidransky and Jin Jen",
year = "2001",
month = "1",
day = "15",
doi = "10.1002/1097-0215(200002)9999:9999<::AID-IJC1031>3.0.CO;2-0",
language = "English (US)",
volume = "91",
pages = "200--204",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Microsatellite instability at AAAG repeat sequences in respiratory tract cancers

AU - Xu, Lihua

AU - Chow, John

AU - Bonacum, Julie

AU - Eisenberger, Claus

AU - Ahrendt, Steve A.

AU - Spafford, Michael

AU - Wu, Li

AU - Lee, Sheng M.

AU - Piantadosi, Steven

AU - Tockman, Melvyn S.

AU - Sidransky, David

AU - Jen, Jin

PY - 2001/1/15

Y1 - 2001/1/15

N2 - We surveyed the occurrence of novel alleles at microsatellite sequences in non-small cell lung cancers (NSCLC) using 61 tetranucleotide repeat markers. The presence of at least one new allele, consistent with microsatellite instability (MSI), was observed in 26 of 61 (43%) markers involving 30 of 47 (64%) NSCLC. Twelve of the 26 markers detected new alleles in 2 or more tumors and 11 of these 12 markers contained an AAAG repeat sequence. Using this panel of 12 markers, MSI was detected in 24 of 47 (51%) NSCLC and 10 of 18 (56%) head and neck cancers but was only observed in 8 of 38 (21%) bladder cancers and 3 of 25 (12%) kidney cancers. Our results suggested that about 50% of respiratory tract cancers exhibited microsatellite instability predominantly at AAAG sequences. This distinct type of instability was termed EMAST for elevated microsatellite alterations at selected tetranucleotide repeats. The identification of markers with EMAST should have potential application for the molecular detection of respiratory tract cancers.

AB - We surveyed the occurrence of novel alleles at microsatellite sequences in non-small cell lung cancers (NSCLC) using 61 tetranucleotide repeat markers. The presence of at least one new allele, consistent with microsatellite instability (MSI), was observed in 26 of 61 (43%) markers involving 30 of 47 (64%) NSCLC. Twelve of the 26 markers detected new alleles in 2 or more tumors and 11 of these 12 markers contained an AAAG repeat sequence. Using this panel of 12 markers, MSI was detected in 24 of 47 (51%) NSCLC and 10 of 18 (56%) head and neck cancers but was only observed in 8 of 38 (21%) bladder cancers and 3 of 25 (12%) kidney cancers. Our results suggested that about 50% of respiratory tract cancers exhibited microsatellite instability predominantly at AAAG sequences. This distinct type of instability was termed EMAST for elevated microsatellite alterations at selected tetranucleotide repeats. The identification of markers with EMAST should have potential application for the molecular detection of respiratory tract cancers.

KW - AAAG repeats

KW - Cancer genetics

KW - Microsatellite instability

KW - NSCLC

KW - Tumor detection

UR - http://www.scopus.com/inward/record.url?scp=17744388968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17744388968&partnerID=8YFLogxK

U2 - 10.1002/1097-0215(200002)9999:9999<::AID-IJC1031>3.0.CO;2-0

DO - 10.1002/1097-0215(200002)9999:9999<::AID-IJC1031>3.0.CO;2-0

M3 - Article

C2 - 11146445

AN - SCOPUS:17744388968

VL - 91

SP - 200

EP - 204

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 2

ER -