MicroRNA-mRNA interactions in a murine model of hyperoxia-induced bronchopulmonary dysplasia

Jie Dong, William A. Carey, Stuart Abel, Christopher Collura, Guoqian D Jiang, Sandra Tomaszek, Shari Sutor, Anja Roden, Yan Asmann, Y.s. Prakash, Dennis A Wigle

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: Bronchopulmonary dysplasia is a chronic lung disease of premature neonates characterized by arrested pulmonary alveolar development. There is increasing evidence that microRNAs (miRNAs) regulate translation of messenger RNAs (mRNAs) during lung organogenesis. The potential role of miRNAs in the pathogenesis of BPD is unclear.Results: Following exposure of neonatal mice to 80% O 2 or room air (RA) for either 14 or 29 days, lungs of hyperoxic mice displayed histological changes consistent with BPD. Comprehensive miRNA and mRNA profiling was performed using lung tissue from both O 2 and RA treated mice, identifying a number of dynamically regulated miRNAs and associated mRNA target genes. Gene ontology enrichment and pathway analysis revealed that hyperoxia modulated genes involved in a variety of lung developmental processes, including cell cycle, cell adhesion, mobility and taxis, inflammation, and angiogenesis. MiR-29 was prominently increased in the lungs of hyperoxic mice, and several predicted mRNA targets of miR-29 were validated with real-time PCR, western blotting and immunohistochemistry. Direct miR-29 targets were further validated in vitro using bronchoalveolar stem cells.Conclusion: In newborn mice, prolonged hyperoxia induces an arrest of alveolar development similar to that seen in human neonates with BPD. This abnormal lung development is accompanied by significant increases in the levels of multiple miRNAs and corresponding decreases in the levels of predicted mRNA targets, many of which have known or suspected roles in pathways altered in BPD. These data support the hypothesis that dynamic regulation of miRNAs plays a prominent role in the pathophysiology of BPD.

Original languageEnglish (US)
Article number204
JournalBMC Genomics
Volume13
Issue number1
DOIs
StatePublished - May 30 2012

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Bronchopulmonary Dysplasia
Hyperoxia
MicroRNAs
Lung
Messenger RNA
Air
Gene Ontology
Organogenesis
Cell Adhesion
Lung Diseases
Genes
Real-Time Polymerase Chain Reaction
Cell Cycle
Chronic Disease
Stem Cells
Western Blotting
Immunohistochemistry
Inflammation

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

MicroRNA-mRNA interactions in a murine model of hyperoxia-induced bronchopulmonary dysplasia. / Dong, Jie; Carey, William A.; Abel, Stuart; Collura, Christopher; Jiang, Guoqian D; Tomaszek, Sandra; Sutor, Shari; Roden, Anja; Asmann, Yan; Prakash, Y.s.; Wigle, Dennis A.

In: BMC Genomics, Vol. 13, No. 1, 204, 30.05.2012.

Research output: Contribution to journalArticle

Dong, Jie ; Carey, William A. ; Abel, Stuart ; Collura, Christopher ; Jiang, Guoqian D ; Tomaszek, Sandra ; Sutor, Shari ; Roden, Anja ; Asmann, Yan ; Prakash, Y.s. ; Wigle, Dennis A. / MicroRNA-mRNA interactions in a murine model of hyperoxia-induced bronchopulmonary dysplasia. In: BMC Genomics. 2012 ; Vol. 13, No. 1.
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abstract = "Background: Bronchopulmonary dysplasia is a chronic lung disease of premature neonates characterized by arrested pulmonary alveolar development. There is increasing evidence that microRNAs (miRNAs) regulate translation of messenger RNAs (mRNAs) during lung organogenesis. The potential role of miRNAs in the pathogenesis of BPD is unclear.Results: Following exposure of neonatal mice to 80{\%} O 2 or room air (RA) for either 14 or 29 days, lungs of hyperoxic mice displayed histological changes consistent with BPD. Comprehensive miRNA and mRNA profiling was performed using lung tissue from both O 2 and RA treated mice, identifying a number of dynamically regulated miRNAs and associated mRNA target genes. Gene ontology enrichment and pathway analysis revealed that hyperoxia modulated genes involved in a variety of lung developmental processes, including cell cycle, cell adhesion, mobility and taxis, inflammation, and angiogenesis. MiR-29 was prominently increased in the lungs of hyperoxic mice, and several predicted mRNA targets of miR-29 were validated with real-time PCR, western blotting and immunohistochemistry. Direct miR-29 targets were further validated in vitro using bronchoalveolar stem cells.Conclusion: In newborn mice, prolonged hyperoxia induces an arrest of alveolar development similar to that seen in human neonates with BPD. This abnormal lung development is accompanied by significant increases in the levels of multiple miRNAs and corresponding decreases in the levels of predicted mRNA targets, many of which have known or suspected roles in pathways altered in BPD. These data support the hypothesis that dynamic regulation of miRNAs plays a prominent role in the pathophysiology of BPD.",
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