microRNA-mediated survivin control of pluripotency

Kristina Kapinas, Heesun Kim, Matthew Mandeville, Lori A. Martin-Buley, Carlo M. Croce, Jane B. Lian, Andre J. Van Wijnen, Janet L. Stein, Dario C. Altieri, Gary S. Stein

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Understanding the mechanisms that sustain pluripotency in human embryonic stem cells (hESCs) is an active area of research that may prove useful in regenerative medicine and will provide fundamental information relevant to development and cancer. hESCs and cancer cells share the unique ability to proliferate indefinitely and rapidly. Because the protein survivin is uniquely overexpressed in virtually all human cancers and in hESCs, we sought to investigate its role in supporting the distinctive capabilities of these cell types. Results presented here suggest that survivin contributes to the maintenance of pluripotency and that post-transcriptional control of survivin isoform expression is selectively regulated by microRNAs. miR-203 has been extensively studied in human tumors, but has not been characterized in hESCs. We show that miR-203 expression and activity is consistent with the expression and subcellular localization of survivin isoforms that in turn modulate expression of the Oct4 and Nanog transcription factors to sustain pluripotency. This study contributes to understanding of the complex regulatory mechanisms that govern whether hESCs proliferate or commit to lineages.

Original languageEnglish (US)
Pages (from-to)63-70
Number of pages8
JournalJournal of Cellular Physiology
Volume230
Issue number1
DOIs
StatePublished - 2015

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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    Kapinas, K., Kim, H., Mandeville, M., Martin-Buley, L. A., Croce, C. M., Lian, J. B., Van Wijnen, A. J., Stein, J. L., Altieri, D. C., & Stein, G. S. (2015). microRNA-mediated survivin control of pluripotency. Journal of Cellular Physiology, 230(1), 63-70. https://doi.org/10.1002/jcp.24681