MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors

Nicole Georgi, Hanna Taipaleenmaki, Christian C. Raiss, Nathalie Groen, Karolina Janaeczek Portalska, Clemens Van Blitterswijk, Jan De Boer, Janine N. Post, Andre J van Wijnen, Marcel Karperien

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ability of human mesenchymal stromal/stem cells (hMSCs) to differentiate into various mesenchymal cell lineages makes them a promising cell source for the use in tissue repair strategies. Since the differentiation potential of hMSCs differs between donors, it is necessary to establish biomarkers for the identification of donors with high differentiation potential. In this study, we show that microRNA (miRNA) expression levels are effective for distinguishing donors with high differentiation potential from low differentiation potential. Twenty hMSC donors were initially tested for marker expression and differentiation potential. In particular, the chondrogenic differentiation potential was evaluated on the basis of histological matrix formation, mRNA expression levels of chondrogenic marker genes, and quantitative glycosaminoglycan deposition. Three donors out of twenty were identified as donors with high chondrogenic potential, whereas nine showed moderate and eight showed low chondrogenic potential. Expression profiles of miRNAs involved in chondrogenesis and cartilage homeostasis were used for the distinction between high-performance hMSCs and low-performance hMSCs. Global mRNA expression profiles of the donors before the onset of chondrogenic differentiation revealed minor differences in gene expression between low and high chondrogenic performers. However, analysis of miRNA expression during a 7-day differentiation period identified miR-210 and miR-630 as positive regulators of chondrogenesis. In contrast, miR-181 and miR-34a, which are negative regulators of chondrogenesis, were upregulated during differentiation in low-performing donors. In conclusion, profiling of hMSC donors for a specific panel of miRNAs may have a prognostic value for selecting donors with high differentiation potential to improve hMSC-based strategies for tissue regeneration.

Original languageEnglish (US)
Pages (from-to)1946-1955
Number of pages10
JournalStem Cells and Development
Volume24
Issue number16
DOIs
StatePublished - Aug 15 2015

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Differentiation Antigens
MicroRNAs
Mesenchymal Stromal Cells
Chondrogenesis
Messenger RNA
Cell Lineage
Glycosaminoglycans
Cartilage
Regeneration
Homeostasis

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Georgi, N., Taipaleenmaki, H., Raiss, C. C., Groen, N., Portalska, K. J., Van Blitterswijk, C., ... Karperien, M. (2015). MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors. Stem Cells and Development, 24(16), 1946-1955. https://doi.org/10.1089/scd.2014.0534

MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors. / Georgi, Nicole; Taipaleenmaki, Hanna; Raiss, Christian C.; Groen, Nathalie; Portalska, Karolina Janaeczek; Van Blitterswijk, Clemens; De Boer, Jan; Post, Janine N.; van Wijnen, Andre J; Karperien, Marcel.

In: Stem Cells and Development, Vol. 24, No. 16, 15.08.2015, p. 1946-1955.

Research output: Contribution to journalArticle

Georgi, N, Taipaleenmaki, H, Raiss, CC, Groen, N, Portalska, KJ, Van Blitterswijk, C, De Boer, J, Post, JN, van Wijnen, AJ & Karperien, M 2015, 'MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors', Stem Cells and Development, vol. 24, no. 16, pp. 1946-1955. https://doi.org/10.1089/scd.2014.0534
Georgi N, Taipaleenmaki H, Raiss CC, Groen N, Portalska KJ, Van Blitterswijk C et al. MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors. Stem Cells and Development. 2015 Aug 15;24(16):1946-1955. https://doi.org/10.1089/scd.2014.0534
Georgi, Nicole ; Taipaleenmaki, Hanna ; Raiss, Christian C. ; Groen, Nathalie ; Portalska, Karolina Janaeczek ; Van Blitterswijk, Clemens ; De Boer, Jan ; Post, Janine N. ; van Wijnen, Andre J ; Karperien, Marcel. / MicroRNA Levels as Prognostic Markers for the Differentiation Potential of Human Mesenchymal Stromal Cell Donors. In: Stem Cells and Development. 2015 ; Vol. 24, No. 16. pp. 1946-1955.
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