MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression

Armaz Aschrafi, Jan M. Verheijen, Peter M. Gordebeke, Nikkie F. Olde Loohuis, Kelly Menting, Amanda Jager, Miklos Palkovits, Bram Geenen, Aron Kos, Gerard J.M. Martens, Jeffrey C. Glennon, Barry B. Kaplan, Balázs Gaszner, Tamas Kozicz

Research output: Contribution to journalArticle

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Abstract

Background: Altered levels of urocortin 1 (Ucn1) in the centrally projecting Edinger-Westphal nucleus (EWcp) of depressed suicide attempters or completers mediate the brain’s response to stress, while the mechanism regulating Ucn1 expression is unknown. We tested the hypothesis that microRNAs (miRNAs), which are vital fine-tuners of gene expression during the brain’s response to stress, have the capacity to modulate Ucn1 expression. Methods: Computational analysis revealed that the Ucn1 3’ untranslated region contained a conserved binding site for miR-326. We examined miR-326 and Ucn1 levels in the EWcp of depressed suicide completers. In addition, we evaluated miR-326 and Ucn1 levels in the serum and the EWcp of a chronic variable mild stress (CVMS) rat model of behavioural despair and after recovery from CVMS, respectively. Gain and loss of miR-326 function experiments examined the regulation of Ucn1 by this miRNA in cultured midbrain neurons. Results: We found reduced miR-326 levels concomitant with elevated Ucn1 levels in the EWcp of depressed suicide completers as well as in the EWcp of CVMS rats. In CVMS rats fully recovered from stress, both serum and EWcp miR-326 levels rebounded to nonstressed levels. While downregulation of miR-326 levels in primary midbrain neurons enhanced Ucn1 expression levels, miR-326 overexpression selectively reduced the levels of this neuropeptide. Limitations: This study lacked experiments showing that in vivo alteration of miR-326 levels alleviate depression-like behaviours. We show only correlative data for miR-325 and cocaine- and amphetamine-regulated transcript levels in the EWcp. Conclusion: We identified miR-326 dysregulation in depressed suicide completers and characterized this miRNA as an upstream regulator of the Ucn1 neuropeptide expression in midbrain neurons.

Original languageEnglish (US)
Pages (from-to)342-353
Number of pages12
JournalJournal of Psychiatry and Neuroscience
Volume41
Issue number5
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

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Urocortins
Mesencephalon
MicroRNAs
Suicide
Neuropeptides
Neurons
Brain
3' Untranslated Regions
Amphetamine
Serum
Cocaine

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry
  • Pharmacology (medical)

Cite this

Aschrafi, A., Verheijen, J. M., Gordebeke, P. M., Olde Loohuis, N. F., Menting, K., Jager, A., ... Kozicz, T. (2016). MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression. Journal of Psychiatry and Neuroscience, 41(5), 342-353. https://doi.org/10.1503/jpn.150154

MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression. / Aschrafi, Armaz; Verheijen, Jan M.; Gordebeke, Peter M.; Olde Loohuis, Nikkie F.; Menting, Kelly; Jager, Amanda; Palkovits, Miklos; Geenen, Bram; Kos, Aron; Martens, Gerard J.M.; Glennon, Jeffrey C.; Kaplan, Barry B.; Gaszner, Balázs; Kozicz, Tamas.

In: Journal of Psychiatry and Neuroscience, Vol. 41, No. 5, 01.09.2016, p. 342-353.

Research output: Contribution to journalArticle

Aschrafi, A, Verheijen, JM, Gordebeke, PM, Olde Loohuis, NF, Menting, K, Jager, A, Palkovits, M, Geenen, B, Kos, A, Martens, GJM, Glennon, JC, Kaplan, BB, Gaszner, B & Kozicz, T 2016, 'MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression', Journal of Psychiatry and Neuroscience, vol. 41, no. 5, pp. 342-353. https://doi.org/10.1503/jpn.150154
Aschrafi A, Verheijen JM, Gordebeke PM, Olde Loohuis NF, Menting K, Jager A et al. MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression. Journal of Psychiatry and Neuroscience. 2016 Sep 1;41(5):342-353. https://doi.org/10.1503/jpn.150154
Aschrafi, Armaz ; Verheijen, Jan M. ; Gordebeke, Peter M. ; Olde Loohuis, Nikkie F. ; Menting, Kelly ; Jager, Amanda ; Palkovits, Miklos ; Geenen, Bram ; Kos, Aron ; Martens, Gerard J.M. ; Glennon, Jeffrey C. ; Kaplan, Barry B. ; Gaszner, Balázs ; Kozicz, Tamas. / MicroRNA-326 acts as a molecular switch in the regulation of midbrain urocortin 1 expression. In: Journal of Psychiatry and Neuroscience. 2016 ; Vol. 41, No. 5. pp. 342-353.
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abstract = "Background: Altered levels of urocortin 1 (Ucn1) in the centrally projecting Edinger-Westphal nucleus (EWcp) of depressed suicide attempters or completers mediate the brain’s response to stress, while the mechanism regulating Ucn1 expression is unknown. We tested the hypothesis that microRNAs (miRNAs), which are vital fine-tuners of gene expression during the brain’s response to stress, have the capacity to modulate Ucn1 expression. Methods: Computational analysis revealed that the Ucn1 3’ untranslated region contained a conserved binding site for miR-326. We examined miR-326 and Ucn1 levels in the EWcp of depressed suicide completers. In addition, we evaluated miR-326 and Ucn1 levels in the serum and the EWcp of a chronic variable mild stress (CVMS) rat model of behavioural despair and after recovery from CVMS, respectively. Gain and loss of miR-326 function experiments examined the regulation of Ucn1 by this miRNA in cultured midbrain neurons. Results: We found reduced miR-326 levels concomitant with elevated Ucn1 levels in the EWcp of depressed suicide completers as well as in the EWcp of CVMS rats. In CVMS rats fully recovered from stress, both serum and EWcp miR-326 levels rebounded to nonstressed levels. While downregulation of miR-326 levels in primary midbrain neurons enhanced Ucn1 expression levels, miR-326 overexpression selectively reduced the levels of this neuropeptide. Limitations: This study lacked experiments showing that in vivo alteration of miR-326 levels alleviate depression-like behaviours. We show only correlative data for miR-325 and cocaine- and amphetamine-regulated transcript levels in the EWcp. Conclusion: We identified miR-326 dysregulation in depressed suicide completers and characterized this miRNA as an upstream regulator of the Ucn1 neuropeptide expression in midbrain neurons.",
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AU - Olde Loohuis, Nikkie F.

AU - Menting, Kelly

AU - Jager, Amanda

AU - Palkovits, Miklos

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AU - Kos, Aron

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