MicroRNA-205 inhibits tumor cell migration through down-regulating the expression of the LDL receptor-related protein 1

Heesang Song; Guojun Bu

doi:10.1016/j.bbrc.2009.08.020

MicroRNA-205 inhibits tumor cell migration through down-regulating the expression of the LDL receptor-related protein 1

Heesang Song, Guojun Bu

Neuroscience

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional endocytic receptor that plays critical roles in the pathogenesis of several human diseases including tumor metastasis and Alzheimer's disease. However, mechanisms that regulate LRP1 expression under physiological and pathophysiological conditions are not unclear. In human cell lines, we found that miR-205 down-regulates the expression of LRP1 by targeting sequences in the 3′UTR of LRP1 mRNA. This effect was abolished by deleting the miR-205 seed site in the 3′UTR of LRP1. The ectopic expression of miR-205 also significantly mitigated migration of both U87 and SK-LU-1 cells. These results, for the first time, demonstrate that expression of human LRP1 is regulated in part by a specific miRNA, leading to decreased tumor cell migration.

Original language	English (US)
Pages (from-to)	400-405
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	388
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2009.08.020
State	Published - Oct 16 2009

Keywords

Cell migration
LRP1
MicroRNA
Tumor

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2009.08.020

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title = "MicroRNA-205 inhibits tumor cell migration through down-regulating the expression of the LDL receptor-related protein 1",

abstract = "Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional endocytic receptor that plays critical roles in the pathogenesis of several human diseases including tumor metastasis and Alzheimer's disease. However, mechanisms that regulate LRP1 expression under physiological and pathophysiological conditions are not unclear. In human cell lines, we found that miR-205 down-regulates the expression of LRP1 by targeting sequences in the 3′UTR of LRP1 mRNA. This effect was abolished by deleting the miR-205 seed site in the 3′UTR of LRP1. The ectopic expression of miR-205 also significantly mitigated migration of both U87 and SK-LU-1 cells. These results, for the first time, demonstrate that expression of human LRP1 is regulated in part by a specific miRNA, leading to decreased tumor cell migration.",

keywords = "Cell migration, LRP1, MicroRNA, Tumor",

author = "Heesang Song and Guojun Bu",

note = "Funding Information: We thank Kathleen Baysac for careful reading of this manuscript. This work was supported by grants from the National Institutes of Health R01-AG027924 and R01-AG031784 .",

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AU - Song, Heesang

AU - Bu, Guojun

N1 - Funding Information: We thank Kathleen Baysac for careful reading of this manuscript. This work was supported by grants from the National Institutes of Health R01-AG027924 and R01-AG031784 .

PY - 2009/10/16

Y1 - 2009/10/16

N2 - Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional endocytic receptor that plays critical roles in the pathogenesis of several human diseases including tumor metastasis and Alzheimer's disease. However, mechanisms that regulate LRP1 expression under physiological and pathophysiological conditions are not unclear. In human cell lines, we found that miR-205 down-regulates the expression of LRP1 by targeting sequences in the 3′UTR of LRP1 mRNA. This effect was abolished by deleting the miR-205 seed site in the 3′UTR of LRP1. The ectopic expression of miR-205 also significantly mitigated migration of both U87 and SK-LU-1 cells. These results, for the first time, demonstrate that expression of human LRP1 is regulated in part by a specific miRNA, leading to decreased tumor cell migration.

AB - Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional endocytic receptor that plays critical roles in the pathogenesis of several human diseases including tumor metastasis and Alzheimer's disease. However, mechanisms that regulate LRP1 expression under physiological and pathophysiological conditions are not unclear. In human cell lines, we found that miR-205 down-regulates the expression of LRP1 by targeting sequences in the 3′UTR of LRP1 mRNA. This effect was abolished by deleting the miR-205 seed site in the 3′UTR of LRP1. The ectopic expression of miR-205 also significantly mitigated migration of both U87 and SK-LU-1 cells. These results, for the first time, demonstrate that expression of human LRP1 is regulated in part by a specific miRNA, leading to decreased tumor cell migration.

KW - Cell migration

KW - LRP1

KW - MicroRNA

KW - Tumor

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MicroRNA-205 inhibits tumor cell migration through down-regulating the expression of the LDL receptor-related protein 1

Abstract

Keywords

ASJC Scopus subject areas

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