Microfluidic device for long-term ftir spectromicroscopy of live adherent cells

Kevin Loutherback, Liang Chen, Hoi Ying Holman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

FTIR spectromicroscopy is a label-free, nondestructive analytical technique that can provide spatiotemporal information of biomolecules. Performing FTIR measurements on live cells is challenging due to water's strong IR absorption coupled with a need to provide nutrients in liquid. We developed an open-channel device based on a gold-coated porous membrane that allows long-term, continuous IR measurement of adherent cells; subsequently, we tested this device by observing in real-time a living PC12 cell colony for 6.6 days. This technique is promising for noninvasive studies of mammalian systems, capable of providing insight to disease progression, drug discovery, and even cell regulation and development.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages621-623
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Infrared
  • Mammalian cell culture
  • Microscopy
  • Spectroscopy

ASJC Scopus subject areas

  • Control and Systems Engineering

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