Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels

Scott L. Nyberg; Steven M. Santilli; Russell A. Shatford; Frank B. Cerra; David R. Knighton; Wei Shou Hu

doi:10.1007/BF00155491

Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels

Scott L. Nyberg, Steven M. Santilli, Russell A. Shatford, Frank B. Cerra, David R. Knighton, Wei Shou Hu

Transplant Surgery

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8 Scopus citations

Abstract

Microelectrodes have been used for the measurement of oxygen tension in various biological systems.(Silver, 1987) Although not previously reported, microelectrodes allow the direct measurement of oxygen tension profiles within collagen gels containing entrapped hepatocytes (collagcn-hepatocyte gels). These oxygen tension profiles, along with hepatocyte oxygen consumption data, allowed the estimation of a diffusion coefficient for oxygen in collagen-hepatocyte gels, D_g = 2.99 × 10^-5 cm²/s.

Original language	English (US)
Pages (from-to)	449-452
Number of pages	4
Journal	Biotechnology Techniques
Volume	5
Issue number	6
DOIs	https://doi.org/10.1007/BF00155491
State	Published - Nov 1991

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

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10.1007/BF00155491

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title = "Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels",

abstract = "Microelectrodes have been used for the measurement of oxygen tension in various biological systems.(Silver, 1987) Although not previously reported, microelectrodes allow the direct measurement of oxygen tension profiles within collagen gels containing entrapped hepatocytes (collagcn-hepatocyte gels). These oxygen tension profiles, along with hepatocyte oxygen consumption data, allowed the estimation of a diffusion coefficient for oxygen in collagen-hepatocyte gels, Dg = 2.99 × 10-5 cm2/s.",

author = "Nyberg, {Scott L.} and Santilli, {Steven M.} and Shatford, {Russell A.} and Cerra, {Frank B.} and Knighton, {David R.} and Hu, {Wei Shou}",

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AU - Nyberg, Scott L.

AU - Santilli, Steven M.

AU - Shatford, Russell A.

AU - Cerra, Frank B.

AU - Knighton, David R.

AU - Hu, Wei Shou

PY - 1991/11

Y1 - 1991/11

N2 - Microelectrodes have been used for the measurement of oxygen tension in various biological systems.(Silver, 1987) Although not previously reported, microelectrodes allow the direct measurement of oxygen tension profiles within collagen gels containing entrapped hepatocytes (collagcn-hepatocyte gels). These oxygen tension profiles, along with hepatocyte oxygen consumption data, allowed the estimation of a diffusion coefficient for oxygen in collagen-hepatocyte gels, Dg = 2.99 × 10-5 cm2/s.

AB - Microelectrodes have been used for the measurement of oxygen tension in various biological systems.(Silver, 1987) Although not previously reported, microelectrodes allow the direct measurement of oxygen tension profiles within collagen gels containing entrapped hepatocytes (collagcn-hepatocyte gels). These oxygen tension profiles, along with hepatocyte oxygen consumption data, allowed the estimation of a diffusion coefficient for oxygen in collagen-hepatocyte gels, Dg = 2.99 × 10-5 cm2/s.

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ER -

Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels

Abstract

ASJC Scopus subject areas

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