Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels

Scott L. Nyberg, Steven M. Santilli, Russell A. Shatford, Frank B. Cerra, David R. Knighton, Wei Shou Hu

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Microelectrodes have been used for the measurement of oxygen tension in various biological systems.(Silver, 1987) Although not previously reported, microelectrodes allow the direct measurement of oxygen tension profiles within collagen gels containing entrapped hepatocytes (collagcn-hepatocyte gels). These oxygen tension profiles, along with hepatocyte oxygen consumption data, allowed the estimation of a diffusion coefficient for oxygen in collagen-hepatocyte gels, Dg = 2.99 × 10-5 cm2/s.

Original languageEnglish (US)
Pages (from-to)449-452
Number of pages4
JournalBiotechnology Techniques
Volume5
Issue number6
DOIs
StatePublished - Nov 1 1991

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ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Nyberg, S. L., Santilli, S. M., Shatford, R. A., Cerra, F. B., Knighton, D. R., & Hu, W. S. (1991). Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels. Biotechnology Techniques, 5(6), 449-452. https://doi.org/10.1007/BF00155491