Abstract
The rice aneuploids with telochromosomes are ideal genetic stocks for chromosome arm identification and microdissection. A rice telo-tetrasomic line with two extra short arms of chromosome 5 (5S) was used in the present study. The arms of 5S were microdissected from the prometaphase cells in mitosis and amplified with linker adaptor PCR (LA-PCR). The amplified fragments, ranging from 200-3000 bp, were confirmed to be from the rice chromosome arm 5S by the rice STS and microsatellite markers.
Original language | English (US) |
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Pages (from-to) | 590-594 |
Number of pages | 5 |
Journal | Chinese Science Bulletin |
Volume | 43 |
Issue number | 7 |
DOIs | |
State | Published - Apr 1998 |
Keywords
- Chromosome arm
- Microdissection
- Rice
- Telo-tetrasomic
ASJC Scopus subject areas
- General