Microdissection and amplification of the chromosome arm 5S in a rice telo-tetrasomic

Zhukuan Cheng, Huihuang Yan, Benyuan Dang, Zanmin Hu, Minghong Gu, Lihuang Zhu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The rice aneuploids with telochromosomes are ideal genetic stocks for chromosome arm identification and microdissection. A rice telo-tetrasomic line with two extra short arms of chromosome 5 (5S) was used in the present study. The arms of 5S were microdissected from the prometaphase cells in mitosis and amplified with linker adaptor PCR (LA-PCR). The amplified fragments, ranging from 200-3000 bp, were confirmed to be from the rice chromosome arm 5S by the rice STS and microsatellite markers.

Original languageEnglish (US)
Pages (from-to)590-594
Number of pages5
JournalChinese Science Bulletin
Volume43
Issue number7
DOIs
StatePublished - Apr 1998

Keywords

  • Chromosome arm
  • Microdissection
  • Rice
  • Telo-tetrasomic

ASJC Scopus subject areas

  • General

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