TY - JOUR
T1 - Microcomputed tomography of kidneys following chronic bile duct ligation
AU - Ortiz, M. Clara
AU - García-sanz, AgustíN
AU - Bentley, Michael D.
AU - Fortepiani, Lourdes A.
AU - García-Estañ, JoaquíN
AU - Ritman, Erik L.
AU - Romero, J. Carlos
AU - Juncos, Luis A.
N1 - Funding Information:
This work was supported, in part, by NSF grant BIR9317816, NIH grants RR11800 and HL16496, and SAF97-0176 from the Spanish Health and Pharmacy National Plan (J.G.E.). Dr. Ortiz was supported by a fellowship of the Spanish Society of Hypertension donated by Merck Sharp & Dohme. Dr. García-Sanz was supported by the National Committee for the Development of Science and Technology of Venezuela (CONICIT) and Elmor Laboratories. The authors are grateful to Mr. Steven Jorgensen, Ms. Patricia Lund, and Ms. Denize Reyes for their technical assistance; to Ms. Kristy Zodrow for preparation of this manuscript; and to Ms. Julie Patterson for preparation of the illustrations.
PY - 2000
Y1 - 2000
N2 - Background. In hepatic cirrhosis, renal sodium and water retention can occur prior to decreases in renal blood flow (RBF). This may be explained in part by redistribution of the intrarenal microcirculation toward the juxtamedullary nephrons. To appreciate this three-dimensional spatial redistribution better, we examined the intrarenal microcirculatory changes using microcomputed tomography (micro-CT) in rats subjected to chronic bile duct ligation (CBDL). Methods. Six kidneys from control rats and eight kidneys from rats that had undergone CBDL for 21 days were perfusion fixed in situ at physiological pressure, perfused with silicon-based Microfil® containing lead chromate, embedded in plastic, and scanned by micro-CT. The microvasculature in the reconstructed three-dimensional renal images was studied using computerized image-analysis techniques. To determine the physiological condition of the rats, parallel experiments were conducted on six control and six CBDL rats to measure mean arterial pressure (MAP), RBF, glomerular filtration rate (GFR), urine flow (UF) rate, and sodium excretion by conventional methods. Results. The percentage of vasculature in the renal cortex from CBDL rats was significantly decreased (10.8 ± 0.4% vs. 16.8 ± 2.7% control values). However, the vascular volume fractions of the medullary tissues were not significantly altered. There were no significant differences in the number of glomeruli between groups (36,430 ± 1908 CBDLs, 36,609 ± 3167 controls). The CBDL rats had a similar GFR than the controls but a reduced MAP, RBF, UF, and sodium excretion. Conclusions. The results indicate that after CBDL, there is a selective decrease in cortical vascular filling, which may contribute to the salt and water retention that accompanies cirrhosis.
AB - Background. In hepatic cirrhosis, renal sodium and water retention can occur prior to decreases in renal blood flow (RBF). This may be explained in part by redistribution of the intrarenal microcirculation toward the juxtamedullary nephrons. To appreciate this three-dimensional spatial redistribution better, we examined the intrarenal microcirculatory changes using microcomputed tomography (micro-CT) in rats subjected to chronic bile duct ligation (CBDL). Methods. Six kidneys from control rats and eight kidneys from rats that had undergone CBDL for 21 days were perfusion fixed in situ at physiological pressure, perfused with silicon-based Microfil® containing lead chromate, embedded in plastic, and scanned by micro-CT. The microvasculature in the reconstructed three-dimensional renal images was studied using computerized image-analysis techniques. To determine the physiological condition of the rats, parallel experiments were conducted on six control and six CBDL rats to measure mean arterial pressure (MAP), RBF, glomerular filtration rate (GFR), urine flow (UF) rate, and sodium excretion by conventional methods. Results. The percentage of vasculature in the renal cortex from CBDL rats was significantly decreased (10.8 ± 0.4% vs. 16.8 ± 2.7% control values). However, the vascular volume fractions of the medullary tissues were not significantly altered. There were no significant differences in the number of glomeruli between groups (36,430 ± 1908 CBDLs, 36,609 ± 3167 controls). The CBDL rats had a similar GFR than the controls but a reduced MAP, RBF, UF, and sodium excretion. Conclusions. The results indicate that after CBDL, there is a selective decrease in cortical vascular filling, which may contribute to the salt and water retention that accompanies cirrhosis.
KW - Blood flow redistribution
KW - Hepatic cirrhosis
KW - Hepatorenal syndrome
KW - Kidney
KW - Renal microcirculation
KW - Sodium retention
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U2 - 10.1046/j.1523-1755.2000.00324.x
DO - 10.1046/j.1523-1755.2000.00324.x
M3 - Article
C2 - 11012897
AN - SCOPUS:0033806559
SN - 0085-2538
VL - 58
SP - 1632
EP - 1640
JO - Kidney international
JF - Kidney international
IS - 4
ER -