Microbial contamination of harvested bone marrow and peripheral blood

H. M. Prince, S. R. Page, A. Keating, R. F. Saragosa, N. M. Vukovic, K. R. Imrie, M. Crump, Alexander Keith Stewart

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The incidence and clinical consequences of microbiological contamination of autologous bone marrow and peripheral blood progenitor cells are not well documented. We therefore retrospectively analysed our experience with bacterial or fungal contamination of harvested bone marrow and/or peripheral blood. From January 1987 to the end of January 1994, 499 patients mere harvested or which 301 were transplanted. A total of 3910 specimens obtained during three stages in the processing were assessed for microbial contamination: (1) in the operating room immediately after harvesting (1662 bags) with 2.1% culture positivity, (2) after processing for cryopreservation (1039 bags) with a further 1.1% cultures positive, and (3) after thawing at the time of reinfusion (1209 bags) of which 2.2% mere culture positive. There were no culture positive specimens obtained from any peripheral blood progenitor cell products. The vast majority of the 85 culture positive specimens obtained from marrow were skin flora (89%) and 35% of all positive harvest specimens remained positive following processing and freezing. At least 36% of culture positive specimens were thought to have arisen as a result of exogenous contamination of blood culture bottles. Potentially pathogenic enteric organisms were present in nine (0.2%) specimens and infusion of these organisms occurred in four cases. A further seven patients were reinfused with marrow culture positive for skin organisms. No adverse clinical sequelae were noted following infusion of any contaminated products. However, clinical decision making continues to be influenced by culture results and multistage microbial culture continues to be of value in the management of our marrow recipients.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalBone Marrow Transplantation
Volume15
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Bone Marrow
Blood Cells
Stem Cells
Skin
Cryopreservation
Operating Rooms
Freezing
Incidence

Keywords

  • Blood progenitor cells
  • Bone marrow
  • Microbial contamination

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Prince, H. M., Page, S. R., Keating, A., Saragosa, R. F., Vukovic, N. M., Imrie, K. R., ... Stewart, A. K. (1995). Microbial contamination of harvested bone marrow and peripheral blood. Bone Marrow Transplantation, 15(1), 87-91.

Microbial contamination of harvested bone marrow and peripheral blood. / Prince, H. M.; Page, S. R.; Keating, A.; Saragosa, R. F.; Vukovic, N. M.; Imrie, K. R.; Crump, M.; Stewart, Alexander Keith.

In: Bone Marrow Transplantation, Vol. 15, No. 1, 1995, p. 87-91.

Research output: Contribution to journalArticle

Prince, HM, Page, SR, Keating, A, Saragosa, RF, Vukovic, NM, Imrie, KR, Crump, M & Stewart, AK 1995, 'Microbial contamination of harvested bone marrow and peripheral blood', Bone Marrow Transplantation, vol. 15, no. 1, pp. 87-91.
Prince HM, Page SR, Keating A, Saragosa RF, Vukovic NM, Imrie KR et al. Microbial contamination of harvested bone marrow and peripheral blood. Bone Marrow Transplantation. 1995;15(1):87-91.
Prince, H. M. ; Page, S. R. ; Keating, A. ; Saragosa, R. F. ; Vukovic, N. M. ; Imrie, K. R. ; Crump, M. ; Stewart, Alexander Keith. / Microbial contamination of harvested bone marrow and peripheral blood. In: Bone Marrow Transplantation. 1995 ; Vol. 15, No. 1. pp. 87-91.
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