Microarray probes and probe sets

Hongfang D Liu, Ionut Bebu, Xin Li

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

DNA microarrays have gained wide use in biomedical research by simultaneously monitoring the expression levels of a large number of genes. The successful implementation of DNA microarray technologies requires the development of methods and techniques for the fabrication of microarrays, the selection of probes to represent genes, the quantification of hybridization, and data analysis. In this paper, we concentrate on probes that are either spotted or synthesized on the glass slides through several aspects: sources of probes, the criteria for selecting probes, tools available for probe selections, and probes used in commercial microarray chips. We then provide a detailed review of one type of DNA microarray: Affymetrix GeneChips, discuss the need to re-annotate probes, review different methods for regrouping probes into probe sets, and compare various redefinitions through public available datasets.

Original languageEnglish (US)
Pages (from-to)325-338
Number of pages14
JournalFrontiers in Bioscience - Elite
Volume2 E
Issue number1
StatePublished - Jan 1 2010
Externally publishedYes

Fingerprint

Microarrays
Oligonucleotide Array Sequence Analysis
Genes
Glass
Biomedical Research
Technology
DNA
Fabrication
Monitoring

Keywords

  • GeneChips
  • Microarray
  • Probe sets
  • Probes
  • Review

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Liu, H. D., Bebu, I., & Li, X. (2010). Microarray probes and probe sets. Frontiers in Bioscience - Elite, 2 E(1), 325-338.

Microarray probes and probe sets. / Liu, Hongfang D; Bebu, Ionut; Li, Xin.

In: Frontiers in Bioscience - Elite, Vol. 2 E, No. 1, 01.01.2010, p. 325-338.

Research output: Contribution to journalArticle

Liu, HD, Bebu, I & Li, X 2010, 'Microarray probes and probe sets', Frontiers in Bioscience - Elite, vol. 2 E, no. 1, pp. 325-338.
Liu HD, Bebu I, Li X. Microarray probes and probe sets. Frontiers in Bioscience - Elite. 2010 Jan 1;2 E(1):325-338.
Liu, Hongfang D ; Bebu, Ionut ; Li, Xin. / Microarray probes and probe sets. In: Frontiers in Bioscience - Elite. 2010 ; Vol. 2 E, No. 1. pp. 325-338.
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