Microarray analysis of genes associated with cell surface NIS protein levels in breast cancer

Sasha J. Beyer, Xiaoli Zhang, Rafael E Jimenez, Mei Ling T Lee, Andrea L. Richardson, Kun Huang, Sissy M. Jhiang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Na+/I- symporter (NIS)-mediated iodide uptake allows radioiodine therapy for thyroid cancer. NIS is also expressed in breast tumors, raising potential for radionuclide therapy of breast cancer. However, NIS expression in most breast cancers is low and may not be sufficient for radionuclide therapy. We aimed to identify biomarkers associated with NIS expression such that mechanisms underlying NIS modulation in human breast tumors may be elucidated. Methods. Published oligonucleotide microarray data within the National Center for Biotechnology Information Gene Expression Omnibus database were analyzed to identify gene expression tightly correlated with NIS mRNA level among human breast tumors. NIS immunostaining was performed in a tissue microarray composed of 28 human breast tumors which had corresponding oligonucleotide microarray data available for each tumor such that gene expression associated with cell surface NIS protein level could be identified. Results and Discussion. NIS mRNA levels do not vary among breast tumors or when compared to normal breast tissues when detected by Affymetrix oligonucleotide microarray platforms. Cell surface NIS protein levels are much more variable than their corresponding NIS mRNA levels. Despite a limited number of breast tumors examined, our analysis identified cysteinyl-tRNA synthetase as a biomarker that is highly associated with cell surface NIS protein levels in the ER-positive breast cancer subtype. Conclusions: Further investigation on genes associated with cell surface NIS protein levels within each breast cancer molecular subtype may lead to novel targets for selectively increasing NIS expression/function in a subset of breast cancers patients.

Original languageEnglish (US)
Article number397
JournalBMC Research Notes
Volume4
DOIs
StatePublished - 2011

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Symporters
Microarray Analysis
Microarrays
Genes
Breast Neoplasms
Tumors
Proteins
Oligonucleotide Array Sequence Analysis
Gene expression
Oligonucleotides
cysteinyl-tRNA synthetase
Biomarkers
Gene Expression
Radioisotopes
Messenger RNA
Tissue
Information Centers
Iodides
Biotechnology
Set theory

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Microarray analysis of genes associated with cell surface NIS protein levels in breast cancer. / Beyer, Sasha J.; Zhang, Xiaoli; Jimenez, Rafael E; Lee, Mei Ling T; Richardson, Andrea L.; Huang, Kun; Jhiang, Sissy M.

In: BMC Research Notes, Vol. 4, 397, 2011.

Research output: Contribution to journalArticle

Beyer, Sasha J. ; Zhang, Xiaoli ; Jimenez, Rafael E ; Lee, Mei Ling T ; Richardson, Andrea L. ; Huang, Kun ; Jhiang, Sissy M. / Microarray analysis of genes associated with cell surface NIS protein levels in breast cancer. In: BMC Research Notes. 2011 ; Vol. 4.
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