Micro-CT analysis of myocardial blood supply in young and adult rats

Heather M. Schaefer, Patricia E. Beighley, Diane R. Eaker, Andrew J. Vercnocke, Erik L. Ritman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

This study addresses whether the vasculature grows in proportion to the myocardium as the rat heart develops. The volume of myocardium and coronary vessels were estimated from micro-CT images of the hearts injected with Microfil® contrast agent. Young (n=5) and adult (n=5) hearts were scanned, resulting in 3D images comprised of 20?m on-a-side cubic voxels. The myocardial muscle and vessel lumen volumes were measured for all vessels 40 to 320?m in diameter by an erosion and dilation method applied to the binary images in which the contrast in the vessels were assigned '1' and all non-opacified entities were assigned '0'. The average total muscle volume increases by 50%, 129.4 to 237.4mm3, from young to adult rats, while the luminal volume increases by 10%, 16.6 to 18.6mm3. The vessel volume is 12% of the total muscle volume in young and 8% in adults. For a given vessel volume, the muscle volume in the young is 82% of the muscle volume in adults. We conclude that as the heart matures, the myocardium grows more rapidly than the vasculature. This may result in greater angles of separation between vessel branches, and the increase in myocardial coronary volume. The ratio suggests either higher blood flow velocity or a lower metabolic rate in adults.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2009
Subtitle of host publicationBiomedical Applications in Molecular, Structural, and Functional Imaging
DOIs
StatePublished - Jun 22 2009
EventMedical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging - Lake Buena Vista, FL, United States
Duration: Feb 8 2009Feb 10 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7262
ISSN (Print)1605-7422

Other

OtherMedical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging
CountryUnited States
CityLake Buena Vista, FL
Period2/8/092/10/09

Keywords

  • Fractals
  • Maturation
  • Microcirculation
  • Morphological erosion dilation
  • Scaling

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Schaefer, H. M., Beighley, P. E., Eaker, D. R., Vercnocke, A. J., & Ritman, E. L. (2009). Micro-CT analysis of myocardial blood supply in young and adult rats. In Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging [726225] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7262). https://doi.org/10.1117/12.812125