We have demonstrated that, in determining the number of cytons (cell bodies) in a ganglion, it is important to consider the range of sizes of cytons and their associated nucleoli in correcting for the split-cell counting error. We have established the mathematical relationship between nucleolus diameter and cytons diameter microscopically in serial sections. An improved method, utilizing semi-automatic techniques and programmed calculation and plotting, is presented for estimating the volume of the ganglion, the number of cytons of neurons per ganglion, and the corrected frequency distribution of diameters of cytons of neurons.
ASJC Scopus subject areas
- Clinical Neurology