Method of morphometric evaluation of spinal and autonomic ganglia

Kenneth Offord, Michiya Ohta, Roger F. Oenning, Peter James Dyck

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

We have demonstrated that, in determining the number of cytons (cell bodies) in a ganglion, it is important to consider the range of sizes of cytons and their associated nucleoli in correcting for the split-cell counting error. We have established the mathematical relationship between nucleolus diameter and cytons diameter microscopically in serial sections. An improved method, utilizing semi-automatic techniques and programmed calculation and plotting, is presented for estimating the volume of the ganglion, the number of cytons of neurons per ganglion, and the corrected frequency distribution of diameters of cytons of neurons.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of the neurological sciences
Volume22
Issue number1
DOIs
StatePublished - May 1974

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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