Method for measuring mRNA decay rate in saccharomyces cerevisiae

Wenqian Hu, Jeff Coller

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Eukaryotic mRNA degradation is an essential aspect of gene regulation. Properly turning off transcript generation ensures that protein synthesis does not occur indefinitely. By ensuring that all mRNAs are destroyed, cells can adapt quickly to changing physiological and environmental conditions. Eukaryotic cytoplasmic mRNA degradation is predominately initiated by removal of the poly(A) tail at the 3′ end (deadenylation). Following deadenylation, either the mRNA is degraded in a 3′-5′ manner or the cap is removed and the mRNA is degraded 5′-3′ (reviewed in Coller and Parker, 2004). Determining mRNA decay rates, as indicated by mRNA half-life, is vital to understand how mRNA stability is modulated under various physiological conditions.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc
Pages137-155
Number of pages19
ISBN (Print)9780124200371
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume530
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Formaldehyde agarose denaturing gel electrophoresis
  • Northern transfer
  • Radiolabeled oligonucleotide probe
  • Yeast cells
  • mRNA decay rate
  • mRNA half-life calculation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Hu, W., & Coller, J. (2013). Method for measuring mRNA decay rate in saccharomyces cerevisiae. In Laboratory Methods in Enzymology: RNA (pp. 137-155). (Methods in Enzymology; Vol. 530). Academic Press Inc. https://doi.org/10.1016/B978-0-12-420037-1.00007-5