Method for measuring mRNA decay rate in saccharomyces cerevisiae

Wengian Hu, Jeff Coller

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Eukaryotic mRNA degradation is an essential aspect of gene regulation. Properly turning off transcript generation ensures that protein synthesis does not occur indefinitely. By ensuring that all mRNAs are destroyed, cells can adapt quickly to changing physiological and environmental conditions. Eukaryotic cytoplasmic mRNA degradation is predominately initiated by removal of the poly(A) tail at the 3′ end (deadenylation). Following deadenylation, either the mRNA is degraded in a 3′-5′ manner or the cap is removed and the mRNA is degraded 5′-3′ (reviewed in Coller and Parker, 2004). Determining mRNA decay rates, as indicated by mRNA half-life, is vital to understand how mRNA stability is modulated under various physiological conditions.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology: RNA
Pages137-155
Number of pages19
Volume530
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume530
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

RNA Stability
Yeast
Saccharomyces cerevisiae
Messenger RNA
Essential Genes
Half-Life
Degradation
Gene expression
Proteins

Keywords

  • Formaldehyde agarose denaturing gel electrophoresis
  • mRNA decay rate
  • mRNA half-life calculation
  • Northern transfer
  • Radiolabeled oligonucleotide probe
  • Yeast cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Hu, W., & Coller, J. (2013). Method for measuring mRNA decay rate in saccharomyces cerevisiae. In Laboratory Methods in Enzymology: RNA (Vol. 530, pp. 137-155). (Methods in Enzymology; Vol. 530). https://doi.org/10.1016/B978-0-12-420037-1.00007-5

Method for measuring mRNA decay rate in saccharomyces cerevisiae. / Hu, Wengian; Coller, Jeff.

Laboratory Methods in Enzymology: RNA. Vol. 530 2013. p. 137-155 (Methods in Enzymology; Vol. 530).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hu, W & Coller, J 2013, Method for measuring mRNA decay rate in saccharomyces cerevisiae. in Laboratory Methods in Enzymology: RNA. vol. 530, Methods in Enzymology, vol. 530, pp. 137-155. https://doi.org/10.1016/B978-0-12-420037-1.00007-5
Hu W, Coller J. Method for measuring mRNA decay rate in saccharomyces cerevisiae. In Laboratory Methods in Enzymology: RNA. Vol. 530. 2013. p. 137-155. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420037-1.00007-5
Hu, Wengian ; Coller, Jeff. / Method for measuring mRNA decay rate in saccharomyces cerevisiae. Laboratory Methods in Enzymology: RNA. Vol. 530 2013. pp. 137-155 (Methods in Enzymology).
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