Metastasis to sentinel lymph nodes in breast cancer is associated with maturation arrest of dendritic cells and poor co-localization of dendritic cells and CD8 + T cells

Aaron Mansfield, Paivi Heikkila, Karl Von Smitten, Jukka Vakkila, Marjut Leidenius

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The regional immune systems of patients with breast cancer are immunosuppressed. Dendritic cells are professional antigen-presenting cells and present cancer-associated antigens to the adaptive immune system in sentinel lymph nodes. Dendritic cells may promote, or inhibit, an adaptive immune response to specific antigens. Our aim was to assess whether dendritic cells were associated with nodal metastasis in patients with breast cancer. Sentinel lymph nodes of 47 patients with breast cancer with varying degrees of nodal disease and ten controls were evaluated using immunohistochemistry for the accumulation of dendritic cells in general (CD1a +), mature dendritic cells (CD208 +), and plasmacytoid dendritic cells (CD123 +). Cytotoxic T cell and regulatory T cell accumulation were also evaluated. Sentinel lymph nodes with macrometastases demonstrated fewer mature dendritic cells than sentinel lymph nodes without metastasis (p = 0.028), but not controls. There were fewer mature dendritic cells to cytotoxic T cells in sentinel lymph nodes with metastasis than those without (p = 0.033). Also, there were more regulatory T cells to mature dendritic cells in sentinel lymph nodes with metastasis than those without (p = 0.02). In conclusion, our study suggests that sentinel lymph nodes with metastasis have arrest of maturation of dendritic cells, fewer mature dendritic cell interactions with cytotoxic T cells, and more regulatory T cells than sentinel lymph nodes without metastasis in patients with breast cancer. These findings extend our understanding of regional immunosuppression and suggest that most regional immunosuppressive changes are associated with nodal metastasis in breast cancer.

Original languageEnglish (US)
Pages (from-to)391-398
Number of pages8
JournalVirchows Archiv
Volume459
Issue number4
DOIs
StatePublished - Oct 2011
Externally publishedYes

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Dendritic Cells
Breast Neoplasms
Neoplasm Metastasis
T-Lymphocytes
Regulatory T-Lymphocytes
Immune System
Sentinel Lymph Node
Antigens
Adaptive Immunity
Antigen-Presenting Cells
Immunosuppressive Agents
Cell Communication
Immunosuppression
Immunohistochemistry

Keywords

  • Breast cancer
  • Dendritic cells
  • Immunosuppression
  • Lymph nodes
  • Metastasis
  • Regulatory T cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Metastasis to sentinel lymph nodes in breast cancer is associated with maturation arrest of dendritic cells and poor co-localization of dendritic cells and CD8 + T cells. / Mansfield, Aaron; Heikkila, Paivi; Von Smitten, Karl; Vakkila, Jukka; Leidenius, Marjut.

In: Virchows Archiv, Vol. 459, No. 4, 10.2011, p. 391-398.

Research output: Contribution to journalArticle

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abstract = "The regional immune systems of patients with breast cancer are immunosuppressed. Dendritic cells are professional antigen-presenting cells and present cancer-associated antigens to the adaptive immune system in sentinel lymph nodes. Dendritic cells may promote, or inhibit, an adaptive immune response to specific antigens. Our aim was to assess whether dendritic cells were associated with nodal metastasis in patients with breast cancer. Sentinel lymph nodes of 47 patients with breast cancer with varying degrees of nodal disease and ten controls were evaluated using immunohistochemistry for the accumulation of dendritic cells in general (CD1a +), mature dendritic cells (CD208 +), and plasmacytoid dendritic cells (CD123 +). Cytotoxic T cell and regulatory T cell accumulation were also evaluated. Sentinel lymph nodes with macrometastases demonstrated fewer mature dendritic cells than sentinel lymph nodes without metastasis (p = 0.028), but not controls. There were fewer mature dendritic cells to cytotoxic T cells in sentinel lymph nodes with metastasis than those without (p = 0.033). Also, there were more regulatory T cells to mature dendritic cells in sentinel lymph nodes with metastasis than those without (p = 0.02). In conclusion, our study suggests that sentinel lymph nodes with metastasis have arrest of maturation of dendritic cells, fewer mature dendritic cell interactions with cytotoxic T cells, and more regulatory T cells than sentinel lymph nodes without metastasis in patients with breast cancer. These findings extend our understanding of regional immunosuppression and suggest that most regional immunosuppressive changes are associated with nodal metastasis in breast cancer.",
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