Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic leukemia

Kimberly J. Stockero, Stephanie R. Fink, Stephanie A. Smoley, Sarah F. Paternoster, Tait D. Shanafelt, Timothy G. Call, Clive S. Zent, Daniel L. Van Dyke, Neil Elliot Kay, Gordon W. Dewald

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Interphase fluorescence in situ hybridization (FISH) studies with D13S319 show that deletions of 13q14 are common in B-cell chronic lymphocytic leukemia (B-CLL). In contrast, conventional cytogenetic studies in B-CLL seldom reveal abnormalities of chromosome 13. We hypothesized that chromosome 13 anomalies might not be detected because they are caused by cryptic deletions rather than by the absence of dividing B-CLL cells. To investigate this possibility, we used FISH with D13S319 to study metaphases from 12 patients known to have 13q- by interphase FISH. These same patients had normal chromosomes by conventional cytogenetic studies. As a result of this study, we report evidence that B-CLL metaphases with 13q- are not detected because these deletions are often cryptic and not visible by standard G-banding.

Original languageEnglish (US)
Pages (from-to)152-156
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume166
Issue number2
DOIs
StatePublished - Apr 15 2006

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Metaphase
Fluorescence In Situ Hybridization
Chromosomes, Human, Pair 13
Interphase
Cytogenetics
Chromosomes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic leukemia. / Stockero, Kimberly J.; Fink, Stephanie R.; Smoley, Stephanie A.; Paternoster, Sarah F.; Shanafelt, Tait D.; Call, Timothy G.; Zent, Clive S.; Van Dyke, Daniel L.; Kay, Neil Elliot; Dewald, Gordon W.

In: Cancer Genetics and Cytogenetics, Vol. 166, No. 2, 15.04.2006, p. 152-156.

Research output: Contribution to journalArticle

Stockero, Kimberly J. ; Fink, Stephanie R. ; Smoley, Stephanie A. ; Paternoster, Sarah F. ; Shanafelt, Tait D. ; Call, Timothy G. ; Zent, Clive S. ; Van Dyke, Daniel L. ; Kay, Neil Elliot ; Dewald, Gordon W. / Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic leukemia. In: Cancer Genetics and Cytogenetics. 2006 ; Vol. 166, No. 2. pp. 152-156.
@article{7844c9941e6141c7a4d59e63bde11c46,
title = "Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic leukemia",
abstract = "Interphase fluorescence in situ hybridization (FISH) studies with D13S319 show that deletions of 13q14 are common in B-cell chronic lymphocytic leukemia (B-CLL). In contrast, conventional cytogenetic studies in B-CLL seldom reveal abnormalities of chromosome 13. We hypothesized that chromosome 13 anomalies might not be detected because they are caused by cryptic deletions rather than by the absence of dividing B-CLL cells. To investigate this possibility, we used FISH with D13S319 to study metaphases from 12 patients known to have 13q- by interphase FISH. These same patients had normal chromosomes by conventional cytogenetic studies. As a result of this study, we report evidence that B-CLL metaphases with 13q- are not detected because these deletions are often cryptic and not visible by standard G-banding.",
author = "Stockero, {Kimberly J.} and Fink, {Stephanie R.} and Smoley, {Stephanie A.} and Paternoster, {Sarah F.} and Shanafelt, {Tait D.} and Call, {Timothy G.} and Zent, {Clive S.} and {Van Dyke}, {Daniel L.} and Kay, {Neil Elliot} and Dewald, {Gordon W.}",
year = "2006",
month = "4",
day = "15",
doi = "10.1016/j.cancergencyto.2005.10.011",
language = "English (US)",
volume = "166",
pages = "152--156",
journal = "Cancer Genetics and Cytogenetics",
issn = "0165-4608",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic leukemia

AU - Stockero, Kimberly J.

AU - Fink, Stephanie R.

AU - Smoley, Stephanie A.

AU - Paternoster, Sarah F.

AU - Shanafelt, Tait D.

AU - Call, Timothy G.

AU - Zent, Clive S.

AU - Van Dyke, Daniel L.

AU - Kay, Neil Elliot

AU - Dewald, Gordon W.

PY - 2006/4/15

Y1 - 2006/4/15

N2 - Interphase fluorescence in situ hybridization (FISH) studies with D13S319 show that deletions of 13q14 are common in B-cell chronic lymphocytic leukemia (B-CLL). In contrast, conventional cytogenetic studies in B-CLL seldom reveal abnormalities of chromosome 13. We hypothesized that chromosome 13 anomalies might not be detected because they are caused by cryptic deletions rather than by the absence of dividing B-CLL cells. To investigate this possibility, we used FISH with D13S319 to study metaphases from 12 patients known to have 13q- by interphase FISH. These same patients had normal chromosomes by conventional cytogenetic studies. As a result of this study, we report evidence that B-CLL metaphases with 13q- are not detected because these deletions are often cryptic and not visible by standard G-banding.

AB - Interphase fluorescence in situ hybridization (FISH) studies with D13S319 show that deletions of 13q14 are common in B-cell chronic lymphocytic leukemia (B-CLL). In contrast, conventional cytogenetic studies in B-CLL seldom reveal abnormalities of chromosome 13. We hypothesized that chromosome 13 anomalies might not be detected because they are caused by cryptic deletions rather than by the absence of dividing B-CLL cells. To investigate this possibility, we used FISH with D13S319 to study metaphases from 12 patients known to have 13q- by interphase FISH. These same patients had normal chromosomes by conventional cytogenetic studies. As a result of this study, we report evidence that B-CLL metaphases with 13q- are not detected because these deletions are often cryptic and not visible by standard G-banding.

UR - http://www.scopus.com/inward/record.url?scp=33646035528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646035528&partnerID=8YFLogxK

U2 - 10.1016/j.cancergencyto.2005.10.011

DO - 10.1016/j.cancergencyto.2005.10.011

M3 - Article

C2 - 16631472

AN - SCOPUS:33646035528

VL - 166

SP - 152

EP - 156

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

SN - 0165-4608

IS - 2

ER -