Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic leukemia

Kimberly J. Stockero, Stephanie R. Fink, Stephanie A. Smoley, Sarah F. Paternoster, Tait D. Shanafelt, Timothy G. Call, Clive S Zent, Daniel L. Van Dyke, Neil E. Kay, Gordon W. Dewald

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Interphase fluorescence in situ hybridization (FISH) studies with D13S319 show that deletions of 13q14 are common in B-cell chronic lymphocytic leukemia (B-CLL). In contrast, conventional cytogenetic studies in B-CLL seldom reveal abnormalities of chromosome 13. We hypothesized that chromosome 13 anomalies might not be detected because they are caused by cryptic deletions rather than by the absence of dividing B-CLL cells. To investigate this possibility, we used FISH with D13S319 to study metaphases from 12 patients known to have 13q- by interphase FISH. These same patients had normal chromosomes by conventional cytogenetic studies. As a result of this study, we report evidence that B-CLL metaphases with 13q- are not detected because these deletions are often cryptic and not visible by standard G-banding.

Original languageEnglish (US)
Pages (from-to)152-156
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume166
Issue number2
DOIs
StatePublished - Apr 15 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'Metaphase cells with normal G-bands have cryptic interstitial deletions in 13q14 detectable by fluorescence in situ hybridization in B-cell chronic lymphocytic leukemia'. Together they form a unique fingerprint.

  • Cite this