TY - JOUR
T1 - Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia
AU - Vijayvargiya, Prakhar
AU - Feri, Adeline
AU - Mairey, Mathilde
AU - Rouillon, Cécile
AU - Jeraldo, Patricio R.
AU - Garrigos, Zerelda Esquer
AU - Thoendel, Matthew J.
AU - Greenwood-Quaintance, Kerryl E.
AU - Sohail, M. Rizwan
AU - Sampathkumar, Priya
AU - Spychalla, Megan T.
AU - Stewart, A. K.
AU - Patnaik, Mrinal M.
AU - Tande, Aaron J.
AU - Cruveiller, Stéphane
AU - Hannet, Irene
AU - Beurdeley, Pascale
AU - Patel, Robin
N1 - Funding Information:
This work was supported by funding from Mayo Clinic Center for Individualized Medicine (94332019 MB17-IM RFA to PV). URL: https://www.mayo.edu/research/centersprograms/center-individualized-medicine. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2022 Vijayvargiya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2022/6
Y1 - 2022/6
N2 - Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospectively enrolled 20 acute leukemia patients and obtained blood from these patients at three time points: 1) anytime from onset of neutropenia until before development of neutropenic fever, 2) within 24 hours of onset of neutropenic fever, 3) 5–7 days after onset of neutropenic fever. Blood samples underwent sample preparation, sequencing and analysis using the iDTECT® Dx Blood v1® platform (PathoQuest, Paris, France). Clinically relevant viruses or bacteria were detected in three cases each by metagenomic shotgun sequencing and blood cultures, albeit with no concordance between the two. Further optimization of sample preparation methods and sequencing platforms is needed before widespread adoption of this technology into clinical practice.
AB - Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun sequencing based assay for detection of bacteria and viruses in blood samples of patients with febrile neutropenia. We prospectively enrolled 20 acute leukemia patients and obtained blood from these patients at three time points: 1) anytime from onset of neutropenia until before development of neutropenic fever, 2) within 24 hours of onset of neutropenic fever, 3) 5–7 days after onset of neutropenic fever. Blood samples underwent sample preparation, sequencing and analysis using the iDTECT® Dx Blood v1® platform (PathoQuest, Paris, France). Clinically relevant viruses or bacteria were detected in three cases each by metagenomic shotgun sequencing and blood cultures, albeit with no concordance between the two. Further optimization of sample preparation methods and sequencing platforms is needed before widespread adoption of this technology into clinical practice.
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U2 - 10.1371/journal.pone.0269405
DO - 10.1371/journal.pone.0269405
M3 - Article
C2 - 35709201
AN - SCOPUS:85132266867
SN - 1932-6203
VL - 17
JO - PLoS One
JF - PLoS One
IS - 6 6
M1 - e0269405
ER -