Metabolism of ketone bodies by skeletal muscle in starvation and uncontrolled diabetes

J. W. Bailey; M. W. Haymond; J. M. Miles

doi:10.1016/0026-0495(90)90163-7

Metabolism of ketone bodies by skeletal muscle in starvation and uncontrolled diabetes

J. W. Bailey, M. W. Haymond, J. M. Miles

Endocrinology, Diabetes, Metabolism, and Nutrition

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3 Scopus citations

Abstract

Previous studies have suggested that skeletal muscle may be responsible for as much as 25% of ketone body (KB) production in poorly controlled diabetes. In the present studies, acetoacetate (AcAc) and β-hydroxybutyrate production was quantitated in the canine hindlimb from surgically placed arterial and venous catheters in conscious insulin-withdrawn diabetic (n = 5) and 4-day fasted (n = 7) dogs. A two-pool modeling technique, using simultaneous infusions of ¹³C acetoacetate and ¹⁴C β-hydroxybutyrate (βOHB) was employed to quantitate total body and hindlimb KB kinetics. Total KB production was 9.4 and 39.3 μmol · kg^-1 · min^-1 in the fasted and diabetic animals, respectively. Hindlimb KB production was negligible in both groups. The two-pool model estimates of hindlimb KB utilization were similar to the values obtained by an arterial-venous difference calculation. In conclusion, the hindlimb does not contribute to de novo synthesis of KBs in either fasted or diabetic dogs. Since species differences in KB metabolism occur, it is possible that muscle may be a site for KB production in humans.

Original language	English (US)
Pages (from-to)	1039-1043
Number of pages	5
Journal	Metabolism
Volume	39
Issue number	10
DOIs	https://doi.org/10.1016/0026-0495(90)90163-7
State	Published - Oct 1990

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1016/0026-0495(90)90163-7

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title = "Metabolism of ketone bodies by skeletal muscle in starvation and uncontrolled diabetes",

abstract = "Previous studies have suggested that skeletal muscle may be responsible for as much as 25% of ketone body (KB) production in poorly controlled diabetes. In the present studies, acetoacetate (AcAc) and β-hydroxybutyrate production was quantitated in the canine hindlimb from surgically placed arterial and venous catheters in conscious insulin-withdrawn diabetic (n = 5) and 4-day fasted (n = 7) dogs. A two-pool modeling technique, using simultaneous infusions of 13C acetoacetate and 14C β-hydroxybutyrate (βOHB) was employed to quantitate total body and hindlimb KB kinetics. Total KB production was 9.4 and 39.3 μmol · kg-1 · min-1 in the fasted and diabetic animals, respectively. Hindlimb KB production was negligible in both groups. The two-pool model estimates of hindlimb KB utilization were similar to the values obtained by an arterial-venous difference calculation. In conclusion, the hindlimb does not contribute to de novo synthesis of KBs in either fasted or diabetic dogs. Since species differences in KB metabolism occur, it is possible that muscle may be a site for KB production in humans.",

author = "Bailey, {J. W.} and Haymond, {M. W.} and Miles, {J. M.}",

note = "Funding Information: From the Endocrine Research Unit, Departments of Medicine and Pediatrics, Mayo Clinic, Rochester, MN. Supported in part by Grants No. AM 33919 and AM 26989 from the US Public Health Service and by the Ma_vo Foundation. Dr Bailey{\textquoteright}s present address is Nutrition and Food Science Department, University of Tennessee, Knoxville, TN. Address reprint requests to John M. Miles. MD, Mayo Clinic, Endocrine Research Unit, 5-164 W. Joseph Building, Rochester, MN 55905. @ 1990 by W.B. Saunders Company. 0026-0495/90/3910-0007$3,00/O",

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AU - Haymond, M. W.

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N1 - Funding Information: From the Endocrine Research Unit, Departments of Medicine and Pediatrics, Mayo Clinic, Rochester, MN. Supported in part by Grants No. AM 33919 and AM 26989 from the US Public Health Service and by the Ma_vo Foundation. Dr Bailey’s present address is Nutrition and Food Science Department, University of Tennessee, Knoxville, TN. Address reprint requests to John M. Miles. MD, Mayo Clinic, Endocrine Research Unit, 5-164 W. Joseph Building, Rochester, MN 55905. @ 1990 by W.B. Saunders Company. 0026-0495/90/3910-0007$3,00/O

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N2 - Previous studies have suggested that skeletal muscle may be responsible for as much as 25% of ketone body (KB) production in poorly controlled diabetes. In the present studies, acetoacetate (AcAc) and β-hydroxybutyrate production was quantitated in the canine hindlimb from surgically placed arterial and venous catheters in conscious insulin-withdrawn diabetic (n = 5) and 4-day fasted (n = 7) dogs. A two-pool modeling technique, using simultaneous infusions of 13C acetoacetate and 14C β-hydroxybutyrate (βOHB) was employed to quantitate total body and hindlimb KB kinetics. Total KB production was 9.4 and 39.3 μmol · kg-1 · min-1 in the fasted and diabetic animals, respectively. Hindlimb KB production was negligible in both groups. The two-pool model estimates of hindlimb KB utilization were similar to the values obtained by an arterial-venous difference calculation. In conclusion, the hindlimb does not contribute to de novo synthesis of KBs in either fasted or diabetic dogs. Since species differences in KB metabolism occur, it is possible that muscle may be a site for KB production in humans.

AB - Previous studies have suggested that skeletal muscle may be responsible for as much as 25% of ketone body (KB) production in poorly controlled diabetes. In the present studies, acetoacetate (AcAc) and β-hydroxybutyrate production was quantitated in the canine hindlimb from surgically placed arterial and venous catheters in conscious insulin-withdrawn diabetic (n = 5) and 4-day fasted (n = 7) dogs. A two-pool modeling technique, using simultaneous infusions of 13C acetoacetate and 14C β-hydroxybutyrate (βOHB) was employed to quantitate total body and hindlimb KB kinetics. Total KB production was 9.4 and 39.3 μmol · kg-1 · min-1 in the fasted and diabetic animals, respectively. Hindlimb KB production was negligible in both groups. The two-pool model estimates of hindlimb KB utilization were similar to the values obtained by an arterial-venous difference calculation. In conclusion, the hindlimb does not contribute to de novo synthesis of KBs in either fasted or diabetic dogs. Since species differences in KB metabolism occur, it is possible that muscle may be a site for KB production in humans.

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Metabolism of ketone bodies by skeletal muscle in starvation and uncontrolled diabetes

Abstract

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