Metabolic Syndrome Modulates Protein Import into the Mitochondria of Porcine Mesenchymal Stem Cells

Arash Aghajani Nargesi, Xiang Yang Zhu, LaTonya Hickson, Sabena M. Conley, Andre J van Wijnen, Lilach O Lerman, Alfonso Eirin

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells (MSCs) are currently being tested in several clinical trials. Mitochondria regulate many aspects of MSC function. Mitochondrial preproteins are rapidly translated and trafficked into the mitochondrion for assembly in their final destination, but whether coexisting cardiovascular risk factors modulate this process is unknown. We hypothesized that metabolic syndrome (MetS) modulates mitochondrial protein import in porcine MSCs. MSCs were isolated from porcine abdominal adipose tissue after 16 weeks of Lean or MetS diet (n = 5 each). RNA-sequencing was performed and differentially expressed mitochondrial mRNAs and microRNAs were identified and validated. Protein expression of transporters of mitochondrial proteins (presequences and precursors) and their respective substrates were measured. Mitochondrial homeostasis was assessed by Western blot and function by cytochrome-c oxidase-IV activity. Forty-five mitochondrial mRNAs were upregulated and 25 downregulated in MetS-MSCs compared to Lean-MSCs. mRNAs upregulated in MetS-MSCs encoded for precursor proteins, whereas those downregulated encoded for presequences. Micro-RNAs upregulated in MetS-MSCs primarily target mRNAs encoding for presequences. Transporters of precursor proteins and their substrates were also upregulated, associated with changes in mitochondrial homeostasis and dysfunction. MetS interferes with mitochondrial protein import, favoring upregulation of precursor proteins, which might be linked to post-transcriptional regulation of presequences. This in turn alters mitochondrial homeostasis and impairs energy production. Our observations highlight the importance of mitochondria in MSC function and provide a molecular framework for optimization of cell-based strategies as we move towards their clinical application.

Original languageEnglish (US)
JournalStem Cell Reviews and Reports
DOIs
StateAccepted/In press - Jan 1 2018

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Mesenchymal Stromal Cells
Mitochondria
Swine
Protein Precursors
Proteins
Mitochondrial Proteins
Homeostasis
MicroRNAs
Down-Regulation
RNA Sequence Analysis
Abdominal Fat
Messenger RNA
Electron Transport Complex IV
Up-Regulation
Western Blotting
Clinical Trials
Diet

Keywords

  • Metabolic syndrome
  • Mitochondria
  • Protein import
  • RNAseq
  • Stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

Cite this

Metabolic Syndrome Modulates Protein Import into the Mitochondria of Porcine Mesenchymal Stem Cells. / Aghajani Nargesi, Arash; Zhu, Xiang Yang; Hickson, LaTonya; Conley, Sabena M.; van Wijnen, Andre J; Lerman, Lilach O; Eirin, Alfonso.

In: Stem Cell Reviews and Reports, 01.01.2018.

Research output: Contribution to journalArticle

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