Metabolic Syndrome Induces Release of Smaller Extracellular Vesicles from Porcine Mesenchymal Stem Cells

Sabena M. Conley, John E. Shook, Xiang Yang Zhu, Alfonso Eirin, Kyra L. Jordan, John R. Woollard, Busra Isik, La Tonya J. Hickson, Amrutesh S. Puranik, Lilach O. Lerman

Research output: Contribution to journalArticle

Abstract

Mesenchymal stromal/stem cells (MSCs) belong to the endogenous cellular reparative system, and can be used exogenously in cell-based therapy. MSCs release extracellular vesicles (EVs), including exosomes and microvesicles, which mediate some of their therapeutic activity through intercellular communication. We have previously demonstrated that metabolic syndrome (MetS) modifies the cargo packed within swine EV, but whether it influences their phenotypical characteristics remains unclear. This study tested the hypothesis that MetS shifts the size distribution of MSC-derived EVs. Adipose tissue-derived MSC-EV subpopulations from Lean (n = 6) and MetS (n = 6) pigs were characterized for number and size using nanoparticle-tracking analysis, flow cytometry, and transmission electron microscopy. Expression of exosomal genes was determined using next-generation RNA-sequencing (RNA-seq). The number of EV released from Lean and MetS pig MSCs was similar, yet MetS-MSCs yielded a higher proportion of small-size EVs (202.4 ± 17.7 nm vs. 280.3 ± 15.1 nm), consistent with exosomes. RNA-seq showed that their EVs were enriched with exosomal markers. Lysosomal activity remained unaltered in MetS-MSCs. Therefore, MetS alters the size distribution of MSC-derived EVs in favor of exosome release. These observations may reflect MSC injury and membrane recycling in MetS or increased expulsion of waste products, and may have important implications for development of adequate cell-based treatments.

Original languageEnglish (US)
Pages (from-to)1271-1278
Number of pages8
JournalCell Transplantation
Volume28
Issue number9-10
DOIs
StatePublished - Sep 1 2019

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Swine
RNA
Exosomes
Extracellular Vesicles
Flow cytometry
Recycling
Genes
Tissue
Nanoparticles
Transmission electron microscopy
Waste Products
RNA Sequence Analysis
Membranes
Communication
Cell- and Tissue-Based Therapy
Transmission Electron Microscopy

Keywords

  • exosomes
  • extracellular vesicles
  • mesenchymal stem cells
  • Metabolic syndrome

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Metabolic Syndrome Induces Release of Smaller Extracellular Vesicles from Porcine Mesenchymal Stem Cells. / Conley, Sabena M.; Shook, John E.; Zhu, Xiang Yang; Eirin, Alfonso; Jordan, Kyra L.; Woollard, John R.; Isik, Busra; Hickson, La Tonya J.; Puranik, Amrutesh S.; Lerman, Lilach O.

In: Cell Transplantation, Vol. 28, No. 9-10, 01.09.2019, p. 1271-1278.

Research output: Contribution to journalArticle

Conley, Sabena M. ; Shook, John E. ; Zhu, Xiang Yang ; Eirin, Alfonso ; Jordan, Kyra L. ; Woollard, John R. ; Isik, Busra ; Hickson, La Tonya J. ; Puranik, Amrutesh S. ; Lerman, Lilach O. / Metabolic Syndrome Induces Release of Smaller Extracellular Vesicles from Porcine Mesenchymal Stem Cells. In: Cell Transplantation. 2019 ; Vol. 28, No. 9-10. pp. 1271-1278.
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