Metabolic responsiveness to training depends on insulin sensitivity and protein content of exosomes in insulin-resistant males

Maria Apostolopoulou; Lucia Mastrototaro; Sonja Hartwig; Dominik Pesta; Klaus Straßburger; Elisabetta de Filippo; Tomas Jelenik; Yanislava Karusheva; Sofiya Gancheva; Daniel Markgraf; Christian Herder; K. Sreekumaran Nair; Andreas S. Reichert; Stefan Lehr; Karsten Müssig; Hadi Al-Hasani; Julia Szendroedi; Michael Roden

doi:10.1126/sciadv.abi9551

Metabolic responsiveness to training depends on insulin sensitivity and protein content of exosomes in insulin-resistant males

Maria Apostolopoulou, Lucia Mastrototaro, Sonja Hartwig, Dominik Pesta, Klaus Straßburger, Elisabetta de Filippo, Tomas Jelenik, Yanislava Karusheva, Sofiya Gancheva, Daniel Markgraf, Christian Herder, K. Sreekumaran Nair, Andreas S. Reichert, Stefan Lehr, Karsten Müssig, Hadi Al-Hasani, Julia Szendroedi, Michael Roden

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article › peer-review

Abstract

High-intensity interval training (HIIT) improves cardiorespiratory fitness (VO2max), but its impact on metabolism remains unclear. We hypothesized that 12-week HIIT increases insulin sensitivity in males with or without type 2 diabetes [T2D and NDM (nondiabetic humans)]. However, despite identically higher VO2max, mainly insulin-resistant (IR) persons (T2D and IR NDM) showed distinct alterations of circulating small extracellular vesicles (SEVs) along with lower inhibitory metabolic (protein kinase Cϵ activity) or inflammatory (nuclear factor κB) signaling in muscle of T2D or IR NDM, respectively. This is related to the specific alterations in SEV proteome reflecting down-regulation of the phospholipase C pathway (T2D) and up-regulated antioxidant capacity (IR NDM). Thus, SEV cargo may contribute to modulating the individual metabolic responsiveness to exercise training in humans.

Original language	English (US)
Article number	abi9551
Journal	Science Advances
Volume	7
Issue number	41
DOIs	https://doi.org/10.1126/sciadv.abi9551
State	Published - Oct 2021

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Apostolopoulou, M., Mastrototaro, L., Hartwig, S., Pesta, D., Straßburger, K., de Filippo, E., Jelenik, T., Karusheva, Y., Gancheva, S., Markgraf, D., Herder, C., Nair, K. S., Reichert, A. S., Lehr, S., Müssig, K., Al-Hasani, H., Szendroedi, J., & Roden, M. (2021). Metabolic responsiveness to training depends on insulin sensitivity and protein content of exosomes in insulin-resistant males. Science Advances, 7(41), Article abi9551. https://doi.org/10.1126/sciadv.abi9551

Apostolopoulou, M, Mastrototaro, L, Hartwig, S, Pesta, D, Straßburger, K, de Filippo, E, Jelenik, T, Karusheva, Y, Gancheva, S, Markgraf, D, Herder, C, Nair, KS, Reichert, AS, Lehr, S, Müssig, K, Al-Hasani, H, Szendroedi, J & Roden, M 2021, 'Metabolic responsiveness to training depends on insulin sensitivity and protein content of exosomes in insulin-resistant males', Science Advances, vol. 7, no. 41, abi9551. https://doi.org/10.1126/sciadv.abi9551

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abstract = "High-intensity interval training (HIIT) improves cardiorespiratory fitness (VO2max), but its impact on metabolism remains unclear. We hypothesized that 12-week HIIT increases insulin sensitivity in males with or without type 2 diabetes [T2D and NDM (nondiabetic humans)]. However, despite identically higher VO2max, mainly insulin-resistant (IR) persons (T2D and IR NDM) showed distinct alterations of circulating small extracellular vesicles (SEVs) along with lower inhibitory metabolic (protein kinase Cϵ activity) or inflammatory (nuclear factor κB) signaling in muscle of T2D or IR NDM, respectively. This is related to the specific alterations in SEV proteome reflecting down-regulation of the phospholipase C pathway (T2D) and up-regulated antioxidant capacity (IR NDM). Thus, SEV cargo may contribute to modulating the individual metabolic responsiveness to exercise training in humans.",

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AU - Mastrototaro, Lucia

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AU - Pesta, Dominik

AU - Straßburger, Klaus

AU - de Filippo, Elisabetta

AU - Jelenik, Tomas

AU - Karusheva, Yanislava

AU - Gancheva, Sofiya

AU - Markgraf, Daniel

AU - Herder, Christian

AU - Nair, K. Sreekumaran

AU - Reichert, Andreas S.

AU - Lehr, Stefan

AU - Müssig, Karsten

AU - Al-Hasani, Hadi

AU - Szendroedi, Julia

AU - Roden, Michael

PY - 2021/10

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AB - High-intensity interval training (HIIT) improves cardiorespiratory fitness (VO2max), but its impact on metabolism remains unclear. We hypothesized that 12-week HIIT increases insulin sensitivity in males with or without type 2 diabetes [T2D and NDM (nondiabetic humans)]. However, despite identically higher VO2max, mainly insulin-resistant (IR) persons (T2D and IR NDM) showed distinct alterations of circulating small extracellular vesicles (SEVs) along with lower inhibitory metabolic (protein kinase Cϵ activity) or inflammatory (nuclear factor κB) signaling in muscle of T2D or IR NDM, respectively. This is related to the specific alterations in SEV proteome reflecting down-regulation of the phospholipase C pathway (T2D) and up-regulated antioxidant capacity (IR NDM). Thus, SEV cargo may contribute to modulating the individual metabolic responsiveness to exercise training in humans.

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Metabolic responsiveness to training depends on insulin sensitivity and protein content of exosomes in insulin-resistant males

Abstract

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