Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation

Wen Zhi Zhan, Hirofumi Miyata, Y.s. Prakash, Gary C Sieck

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

We hypothesized that metabolic adaptations to muscle inactivity are most pronounced when neurotrophic influence is disrupted. In rat diaphragm muscle (Dia(m)), 2 wk of unilateral denervation or tetrodotoxin nerve blockade resulted in a reduction in succinate dehydrogenase (SDH) activity of type I, IIa, and IIx fibers (~50, 70, and 24%, respectively) and a decrease in SDH variability among fibers (~63%). In contrast, inactivity induced by spinal cord hemisection at C2 (ST) resulted in much less change in SDH activity of type I and IIa fibers (~27 and 24%, respectively) and only an ~30% reduction in SDH variability among fibers. Actomyosin adenosinetriphosphatase (ATPase) activities of type I, IIx, and IIb fibers in denervated and tetrodotoxin-treated Dia(m) were reduced by ~20, 45, and 60%, respectively, and actomyosin ATPase variability among fibers was ~60% lower. In contrast, only actomyosin ATPase activity of type IIb fibers was reduced (~20%) in ST Dia(m). These results suggest that disruption of neurotrophic influence has a greater impact on muscle fiber metabolic properties than inactivity per se.

Original languageEnglish (US)
Pages (from-to)1145-1153
Number of pages9
JournalJournal of Applied Physiology
Volume82
Issue number4
StatePublished - Apr 1997

Fingerprint

Diaphragm
Succinate Dehydrogenase
Muscles
Myosins
Tetrodotoxin
Nerve Block
Denervation
Spinal Cord

Keywords

  • actomyosin adenosinetriphosphatase activity
  • denervation
  • muscle fiber
  • spinal hemisection
  • succinate dehydrogenase activity
  • tetrodotoxin

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation. / Zhan, Wen Zhi; Miyata, Hirofumi; Prakash, Y.s.; Sieck, Gary C.

In: Journal of Applied Physiology, Vol. 82, No. 4, 04.1997, p. 1145-1153.

Research output: Contribution to journalArticle

Zhan, Wen Zhi ; Miyata, Hirofumi ; Prakash, Y.s. ; Sieck, Gary C. / Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation. In: Journal of Applied Physiology. 1997 ; Vol. 82, No. 4. pp. 1145-1153.
@article{3b5dbee76e834ae58e2af91d8177264b,
title = "Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation",
abstract = "We hypothesized that metabolic adaptations to muscle inactivity are most pronounced when neurotrophic influence is disrupted. In rat diaphragm muscle (Dia(m)), 2 wk of unilateral denervation or tetrodotoxin nerve blockade resulted in a reduction in succinate dehydrogenase (SDH) activity of type I, IIa, and IIx fibers (~50, 70, and 24{\%}, respectively) and a decrease in SDH variability among fibers (~63{\%}). In contrast, inactivity induced by spinal cord hemisection at C2 (ST) resulted in much less change in SDH activity of type I and IIa fibers (~27 and 24{\%}, respectively) and only an ~30{\%} reduction in SDH variability among fibers. Actomyosin adenosinetriphosphatase (ATPase) activities of type I, IIx, and IIb fibers in denervated and tetrodotoxin-treated Dia(m) were reduced by ~20, 45, and 60{\%}, respectively, and actomyosin ATPase variability among fibers was ~60{\%} lower. In contrast, only actomyosin ATPase activity of type IIb fibers was reduced (~20{\%}) in ST Dia(m). These results suggest that disruption of neurotrophic influence has a greater impact on muscle fiber metabolic properties than inactivity per se.",
keywords = "actomyosin adenosinetriphosphatase activity, denervation, muscle fiber, spinal hemisection, succinate dehydrogenase activity, tetrodotoxin",
author = "Zhan, {Wen Zhi} and Hirofumi Miyata and Y.s. Prakash and Sieck, {Gary C}",
year = "1997",
month = "4",
language = "English (US)",
volume = "82",
pages = "1145--1153",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation

AU - Zhan, Wen Zhi

AU - Miyata, Hirofumi

AU - Prakash, Y.s.

AU - Sieck, Gary C

PY - 1997/4

Y1 - 1997/4

N2 - We hypothesized that metabolic adaptations to muscle inactivity are most pronounced when neurotrophic influence is disrupted. In rat diaphragm muscle (Dia(m)), 2 wk of unilateral denervation or tetrodotoxin nerve blockade resulted in a reduction in succinate dehydrogenase (SDH) activity of type I, IIa, and IIx fibers (~50, 70, and 24%, respectively) and a decrease in SDH variability among fibers (~63%). In contrast, inactivity induced by spinal cord hemisection at C2 (ST) resulted in much less change in SDH activity of type I and IIa fibers (~27 and 24%, respectively) and only an ~30% reduction in SDH variability among fibers. Actomyosin adenosinetriphosphatase (ATPase) activities of type I, IIx, and IIb fibers in denervated and tetrodotoxin-treated Dia(m) were reduced by ~20, 45, and 60%, respectively, and actomyosin ATPase variability among fibers was ~60% lower. In contrast, only actomyosin ATPase activity of type IIb fibers was reduced (~20%) in ST Dia(m). These results suggest that disruption of neurotrophic influence has a greater impact on muscle fiber metabolic properties than inactivity per se.

AB - We hypothesized that metabolic adaptations to muscle inactivity are most pronounced when neurotrophic influence is disrupted. In rat diaphragm muscle (Dia(m)), 2 wk of unilateral denervation or tetrodotoxin nerve blockade resulted in a reduction in succinate dehydrogenase (SDH) activity of type I, IIa, and IIx fibers (~50, 70, and 24%, respectively) and a decrease in SDH variability among fibers (~63%). In contrast, inactivity induced by spinal cord hemisection at C2 (ST) resulted in much less change in SDH activity of type I and IIa fibers (~27 and 24%, respectively) and only an ~30% reduction in SDH variability among fibers. Actomyosin adenosinetriphosphatase (ATPase) activities of type I, IIx, and IIb fibers in denervated and tetrodotoxin-treated Dia(m) were reduced by ~20, 45, and 60%, respectively, and actomyosin ATPase variability among fibers was ~60% lower. In contrast, only actomyosin ATPase activity of type IIb fibers was reduced (~20%) in ST Dia(m). These results suggest that disruption of neurotrophic influence has a greater impact on muscle fiber metabolic properties than inactivity per se.

KW - actomyosin adenosinetriphosphatase activity

KW - denervation

KW - muscle fiber

KW - spinal hemisection

KW - succinate dehydrogenase activity

KW - tetrodotoxin

UR - http://www.scopus.com/inward/record.url?scp=0030900652&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030900652&partnerID=8YFLogxK

M3 - Article

C2 - 9104851

AN - SCOPUS:0030900652

VL - 82

SP - 1145

EP - 1153

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 4

ER -