Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium

Elizabeth A. Stewart, Marine Sahakian, Alan Rhoades, Bradley J. Van Voorhis, Romana A. Nowak

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Objective: To determine whether messenger RNA for the gonadal LH/hCG receptor is present in human endometrium with the use of reverse- transcriptase polymerase chain reaction. Design: In vitro experiment. Setting: Academic medical center. Patient(s): Premenopausal women who were not receiving hormonally active medications and who were undergoing hysterectomy for uterine leiomyomas, menorrhagia, pelvic pain, or uterine prolapse. Intervention(s): Tissue from hysterectomy specimens was processed for RNA and treated with deoxyribonuclease where appropriate, and RNA was reverse-transcribed to complementary DNA. Main Outcome Measure(s): An appropriately sized band after reverse-transcriptase polymerase chain reaction, followed by sequencing to confirm the results. Result(s): A primer pair that spanned the extracellular domain was unable to amplify receptor complementary DNA from human endometrial tissue. For a primer pair that spanned transmembrane regions 2-6 of the receptor and was contained wholly in exon 11, a 552-base pair fragment was amplified successfully in 19 of 25 human endometrial samples. Conclusion(s): The traditional gonadal LH/hCG receptor does not appear to be present in human endometrial tissue. The presence of a portion of the transmembrane part of the molecule suggests that human endometrium may express a truncated or variant form of the receptor.

Original languageEnglish (US)
Pages (from-to)368-372
Number of pages5
JournalFertility and sterility
Volume71
Issue number2
DOIs
StatePublished - Feb 1 1999

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Keywords

  • Endometrium
  • LH receptor
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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