Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium

Elizabeth A Stewart, Marine Sahakian, Alan Rhoades, Bradley J. Van Voorhis, Romana A. Nowak

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective: To determine whether messenger RNA for the gonadal LH/hCG receptor is present in human endometrium with the use of reverse- transcriptase polymerase chain reaction. Design: In vitro experiment. Setting: Academic medical center. Patient(s): Premenopausal women who were not receiving hormonally active medications and who were undergoing hysterectomy for uterine leiomyomas, menorrhagia, pelvic pain, or uterine prolapse. Intervention(s): Tissue from hysterectomy specimens was processed for RNA and treated with deoxyribonuclease where appropriate, and RNA was reverse-transcribed to complementary DNA. Main Outcome Measure(s): An appropriately sized band after reverse-transcriptase polymerase chain reaction, followed by sequencing to confirm the results. Result(s): A primer pair that spanned the extracellular domain was unable to amplify receptor complementary DNA from human endometrial tissue. For a primer pair that spanned transmembrane regions 2-6 of the receptor and was contained wholly in exon 11, a 552-base pair fragment was amplified successfully in 19 of 25 human endometrial samples. Conclusion(s): The traditional gonadal LH/hCG receptor does not appear to be present in human endometrial tissue. The presence of a portion of the transmembrane part of the molecule suggests that human endometrium may express a truncated or variant form of the receptor.

Original languageEnglish (US)
Pages (from-to)368-372
Number of pages5
JournalFertility and Sterility
Volume71
Issue number2
DOIs
StatePublished - Feb 1999
Externally publishedYes

Fingerprint

Gonadal Hormones
LH Receptors
Luteinizing Hormone
Endometrium
RNA
Reverse Transcriptase Polymerase Chain Reaction
Hysterectomy
Complementary DNA
Uterine Prolapse
Menorrhagia
Pelvic Pain
Deoxyribonucleases
Leiomyoma
Base Pairing
Exons
Outcome Assessment (Health Care)
Messenger RNA

Keywords

  • Endometrium
  • LH receptor
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium. / Stewart, Elizabeth A; Sahakian, Marine; Rhoades, Alan; Van Voorhis, Bradley J.; Nowak, Romana A.

In: Fertility and Sterility, Vol. 71, No. 2, 02.1999, p. 368-372.

Research output: Contribution to journalArticle

Stewart, Elizabeth A ; Sahakian, Marine ; Rhoades, Alan ; Van Voorhis, Bradley J. ; Nowak, Romana A. / Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium. In: Fertility and Sterility. 1999 ; Vol. 71, No. 2. pp. 368-372.
@article{958387d7a1834c7db4ab84f231ae1685,
title = "Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium",
abstract = "Objective: To determine whether messenger RNA for the gonadal LH/hCG receptor is present in human endometrium with the use of reverse- transcriptase polymerase chain reaction. Design: In vitro experiment. Setting: Academic medical center. Patient(s): Premenopausal women who were not receiving hormonally active medications and who were undergoing hysterectomy for uterine leiomyomas, menorrhagia, pelvic pain, or uterine prolapse. Intervention(s): Tissue from hysterectomy specimens was processed for RNA and treated with deoxyribonuclease where appropriate, and RNA was reverse-transcribed to complementary DNA. Main Outcome Measure(s): An appropriately sized band after reverse-transcriptase polymerase chain reaction, followed by sequencing to confirm the results. Result(s): A primer pair that spanned the extracellular domain was unable to amplify receptor complementary DNA from human endometrial tissue. For a primer pair that spanned transmembrane regions 2-6 of the receptor and was contained wholly in exon 11, a 552-base pair fragment was amplified successfully in 19 of 25 human endometrial samples. Conclusion(s): The traditional gonadal LH/hCG receptor does not appear to be present in human endometrial tissue. The presence of a portion of the transmembrane part of the molecule suggests that human endometrium may express a truncated or variant form of the receptor.",
keywords = "Endometrium, LH receptor, Polymerase chain reaction",
author = "Stewart, {Elizabeth A} and Marine Sahakian and Alan Rhoades and {Van Voorhis}, {Bradley J.} and Nowak, {Romana A.}",
year = "1999",
month = "2",
doi = "10.1016/S0015-0282(98)00453-1",
language = "English (US)",
volume = "71",
pages = "368--372",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium

AU - Stewart, Elizabeth A

AU - Sahakian, Marine

AU - Rhoades, Alan

AU - Van Voorhis, Bradley J.

AU - Nowak, Romana A.

PY - 1999/2

Y1 - 1999/2

N2 - Objective: To determine whether messenger RNA for the gonadal LH/hCG receptor is present in human endometrium with the use of reverse- transcriptase polymerase chain reaction. Design: In vitro experiment. Setting: Academic medical center. Patient(s): Premenopausal women who were not receiving hormonally active medications and who were undergoing hysterectomy for uterine leiomyomas, menorrhagia, pelvic pain, or uterine prolapse. Intervention(s): Tissue from hysterectomy specimens was processed for RNA and treated with deoxyribonuclease where appropriate, and RNA was reverse-transcribed to complementary DNA. Main Outcome Measure(s): An appropriately sized band after reverse-transcriptase polymerase chain reaction, followed by sequencing to confirm the results. Result(s): A primer pair that spanned the extracellular domain was unable to amplify receptor complementary DNA from human endometrial tissue. For a primer pair that spanned transmembrane regions 2-6 of the receptor and was contained wholly in exon 11, a 552-base pair fragment was amplified successfully in 19 of 25 human endometrial samples. Conclusion(s): The traditional gonadal LH/hCG receptor does not appear to be present in human endometrial tissue. The presence of a portion of the transmembrane part of the molecule suggests that human endometrium may express a truncated or variant form of the receptor.

AB - Objective: To determine whether messenger RNA for the gonadal LH/hCG receptor is present in human endometrium with the use of reverse- transcriptase polymerase chain reaction. Design: In vitro experiment. Setting: Academic medical center. Patient(s): Premenopausal women who were not receiving hormonally active medications and who were undergoing hysterectomy for uterine leiomyomas, menorrhagia, pelvic pain, or uterine prolapse. Intervention(s): Tissue from hysterectomy specimens was processed for RNA and treated with deoxyribonuclease where appropriate, and RNA was reverse-transcribed to complementary DNA. Main Outcome Measure(s): An appropriately sized band after reverse-transcriptase polymerase chain reaction, followed by sequencing to confirm the results. Result(s): A primer pair that spanned the extracellular domain was unable to amplify receptor complementary DNA from human endometrial tissue. For a primer pair that spanned transmembrane regions 2-6 of the receptor and was contained wholly in exon 11, a 552-base pair fragment was amplified successfully in 19 of 25 human endometrial samples. Conclusion(s): The traditional gonadal LH/hCG receptor does not appear to be present in human endometrial tissue. The presence of a portion of the transmembrane part of the molecule suggests that human endometrium may express a truncated or variant form of the receptor.

KW - Endometrium

KW - LH receptor

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0032938473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032938473&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(98)00453-1

DO - 10.1016/S0015-0282(98)00453-1

M3 - Article

C2 - 9988413

AN - SCOPUS:0032938473

VL - 71

SP - 368

EP - 372

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 2

ER -