Meniscus regeneration combining meniscus and mesenchymal stromal cells in a degradable meniscus implant: An in vitro study

M. H. Hagmeijer, L. A. Vonk, M. Fenu, Y. W.A.M. Van Keep, A. J. Krych, Daniel Bart Frederik Saris

Research output: Contribution to journalArticle

Abstract

Meniscus regeneration is an unmet clinical need as damage to the meniscus is common and causes early osteoarthritis. The aim of the present study was to investigate the feasibility of a one-stage cell-based treatment for meniscus regeneration by augmenting a resorbable collagen-based implant with a combination of recycled meniscus cells and mesenchymal stromal cells (MSCs). Cell communication and fate of the different cell types over time in co-culture were evaluated by connexin 43 staining for gap junctions and polymerase chain reaction (PCR) to discriminate between meniscus cells and MSCs, based on a Y-chromosome gene. To define optimal ratios, human meniscus cells and bone-marrow-derived MSCs were cultured in different ratios in cell pellets and type I collagen hydrogels. In addition, cells were seeded on the implant in fibrin glue by static seeding or injection. Cellular communication by gap junctions was shown in co-culture and a decrease in the amount of MSCs over time was demonstrated by PCR. 20: 80 and 10: 90 ratios showed significantly highest glycosaminoglycan and collagen content in collagen hydrogels. The same statistical trend was found in pellet cultures. Significantly more cells were present in the injected implant and cell distribution was more homogenous as compared to the statically seeded implant. The study demonstrated the feasibility of a new one-stage cell-based procedure for meniscus regeneration, using 20% meniscus cells and 80% MSCs seeded statically on the implant. In addition, the stimulatory effect of MSCs towards meniscus cells was demonstrated by communication through gap junctions.

Original languageEnglish (US)
Pages (from-to)51-62
Number of pages12
JournalEuropean Cells and Materials
Volume38
DOIs
StatePublished - Dec 2019

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Mesenchymal Stromal Cells
Collagen
Regeneration
Hydrogels
Polymerase chain reaction
Cellular radio systems
Fibrin Tissue Adhesive
Connexin 43
Communication
Chromosomes
Collagen Type I
Glycosaminoglycans
Gap Junctions
Glues
Bone
Genes
Coculture Techniques
Meniscus
In Vitro Techniques
Y-Linked Genes

Keywords

  • Bone marrow mesenchymal stromal cells
  • Collagen meniscus implant
  • Meniscus cells
  • Meniscus injury
  • Meniscus regeneration
  • Meniscus scaffold

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

Cite this

Meniscus regeneration combining meniscus and mesenchymal stromal cells in a degradable meniscus implant : An in vitro study. / Hagmeijer, M. H.; Vonk, L. A.; Fenu, M.; Van Keep, Y. W.A.M.; Krych, A. J.; Saris, Daniel Bart Frederik.

In: European Cells and Materials, Vol. 38, 12.2019, p. 51-62.

Research output: Contribution to journalArticle

Hagmeijer, M. H. ; Vonk, L. A. ; Fenu, M. ; Van Keep, Y. W.A.M. ; Krych, A. J. ; Saris, Daniel Bart Frederik. / Meniscus regeneration combining meniscus and mesenchymal stromal cells in a degradable meniscus implant : An in vitro study. In: European Cells and Materials. 2019 ; Vol. 38. pp. 51-62.
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