Membrane trafficking of large conductance Ca2+- and voltage-activated K+ (BK) channels is regulated by Rab4 GTPase

Xiao Li Wang, Tong Lu, Xiaojing Sun, Hon Chi Lee

Research output: Contribution to journalArticle

Abstract

The large conductance voltage- and Ca2+-activated K+ (BK) channel is a major ionic determinant of vascular tone, vasodilation, and blood pressure. The activity of BK channels is regulated in part by membrane presentation. Rab GTPase (Rab) regulates important cellular processes, including ion channel membrane trafficking. We hypothesize that Rab4a participates in the regulation of BK channel α-subunit (BK-α) membrane trafficking. We found that vascular BK-α interacts physically with Rab4a. Co-expression of dominant-negative Rab4a reduced BK-α surface expression, whereas that of constitutively-active Rab4a augmented BK-α surface presentation. These novel findings suggest that vascular BK channel membrane expression is regulated by Rab4a through channel membrane trafficking.

Original languageEnglish (US)
Article number118646
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1867
Issue number4
DOIs
StatePublished - Apr 2020

Keywords

  • BK channel
  • Membrane trafficking
  • Rab GTPase
  • Rab4

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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