Abstract
Apoptosis of thymocytes is involved in the negative selection of thymus, but it remains unclear how the cell death of thymocytes is regulated by the thymic stromal cells. By using immunohistochemistry. TdT-mediated dUTP nick end labeling (TUNEL) and flow cytometry methods, it was found that Fas ligand was expressed in the thymic medulla and a mouse thymic medullary type dendritic cell line (MTSC4 ). The DNA fragmentation and TUNEL positive staining of thymocytes were detected in 6 h of cocultures with MTSC4. 97% of the thymocytes which bound to MTSC4 were Fas antigen positive cells. The DNA fragmentation of thymocytes induced by MTSC4 was inhibited by the addition of 20 mmol/L N-acetyl galactosamin monosaccharide and the pretreatment of a monoclonal antibody (PF-18-3) which recognized a putative antigen on MTSC4. These results suggested that the mechanisms of induction of cell death by stromal cells may include the interactions of multiple cell surface molecules, in addition to the Fas/Fas ligand system.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 189-194 |
| Number of pages | 6 |
| Journal | Science in China, Series C: Life Sciences |
| Volume | 41 |
| Issue number | 2 |
| State | Published - 1998 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Fas ligand
- Thymic stromal cell
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agricultural and Biological Sciences (miscellaneous)
Cite this
Membrane molecules in induction of apoptosis of thymocytes by mouse thymic dendritic cells which express Fas ligands. / Dong, Haidong M; Chen, Jing; Chen, Weifeng.
In: Science in China, Series C: Life Sciences, Vol. 41, No. 2, 1998, p. 189-194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Membrane molecules in induction of apoptosis of thymocytes by mouse thymic dendritic cells which express Fas ligands
AU - Dong, Haidong M
AU - Chen, Jing
AU - Chen, Weifeng
PY - 1998
Y1 - 1998
N2 - Apoptosis of thymocytes is involved in the negative selection of thymus, but it remains unclear how the cell death of thymocytes is regulated by the thymic stromal cells. By using immunohistochemistry. TdT-mediated dUTP nick end labeling (TUNEL) and flow cytometry methods, it was found that Fas ligand was expressed in the thymic medulla and a mouse thymic medullary type dendritic cell line (MTSC4 ). The DNA fragmentation and TUNEL positive staining of thymocytes were detected in 6 h of cocultures with MTSC4. 97% of the thymocytes which bound to MTSC4 were Fas antigen positive cells. The DNA fragmentation of thymocytes induced by MTSC4 was inhibited by the addition of 20 mmol/L N-acetyl galactosamin monosaccharide and the pretreatment of a monoclonal antibody (PF-18-3) which recognized a putative antigen on MTSC4. These results suggested that the mechanisms of induction of cell death by stromal cells may include the interactions of multiple cell surface molecules, in addition to the Fas/Fas ligand system.
AB - Apoptosis of thymocytes is involved in the negative selection of thymus, but it remains unclear how the cell death of thymocytes is regulated by the thymic stromal cells. By using immunohistochemistry. TdT-mediated dUTP nick end labeling (TUNEL) and flow cytometry methods, it was found that Fas ligand was expressed in the thymic medulla and a mouse thymic medullary type dendritic cell line (MTSC4 ). The DNA fragmentation and TUNEL positive staining of thymocytes were detected in 6 h of cocultures with MTSC4. 97% of the thymocytes which bound to MTSC4 were Fas antigen positive cells. The DNA fragmentation of thymocytes induced by MTSC4 was inhibited by the addition of 20 mmol/L N-acetyl galactosamin monosaccharide and the pretreatment of a monoclonal antibody (PF-18-3) which recognized a putative antigen on MTSC4. These results suggested that the mechanisms of induction of cell death by stromal cells may include the interactions of multiple cell surface molecules, in addition to the Fas/Fas ligand system.
KW - Apoptosis
KW - Fas ligand
KW - Thymic stromal cell
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M3 - Article
VL - 41
SP - 189
EP - 194
JO - Science in China, Series C: Life Sciences
JF - Science in China, Series C: Life Sciences
SN - 1006-9305
IS - 2
ER -