Membrane molecules determined by the H 2 associated immune response region

Isolation and some properties

S. E. Cullen, C. S. David, D. C. Shreffler, S. G. Nathenson

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Membrane associated molecules that are probably glucoproteins could be specifically precipitated from NP 40 detergent solubilized extracts of radiolabeled mouse spleen or lymph node cells by antisera produced in congenic strain combinations differing only in the Ir gene region which is linked to the H 2 genes. These Ir region products were designated Lna (lymph node antigen) to conform to previous serological work. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of undreduced specific immune precipitates revealed the presence of a possible dimer form, while reduced samples showed only a single peak equivalent to 30,000 daltons. Thus the Lna molecules are clearly distinct from the H 2D and H 2K molecules, which are about 45,000 daltons. Anti Lna antibodies of different specificity can be present in a single serum; there were at least two separate antigen molecules present in one haplotype tested.

Original languageEnglish (US)
Pages (from-to)648-652
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number3
StatePublished - 1974
Externally publishedYes

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Lymph Nodes
Antigens
Membranes
Precipitins
Antibody Specificity
Sodium Dodecyl Sulfate
Detergents
Haplotypes
Genes
Immune Sera
Polyacrylamide Gel Electrophoresis
Spleen
Serum

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Membrane molecules determined by the H 2 associated immune response region : Isolation and some properties. / Cullen, S. E.; David, C. S.; Shreffler, D. C.; Nathenson, S. G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 71, No. 3, 1974, p. 648-652.

Research output: Contribution to journalArticle

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AU - Nathenson, S. G.

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