Membrane cofactor protein (MCP; CD46) expression in transgenic mice

C. Kemper, M. Leung, C. B. Stephensen, C. A. Pinkert, M. K. Liszewski, R. Cattaneo, J. P. Atkinson

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Human membrane cofactor protein (MCP; CD46) is a widely distributed complement regulator. In the mouse, expression of MCP is largely restricted to the testis while a related, widely expressed protein (Crry) appears to perform MCP's (CD46) regulatory activity. We have developed two mouse strains transgenic for human MCP (CD46) utilizing an ∼ 400 kb YAC clone carrying the complete gene. A third mouse strain was generated using an overlapping YAC clone isolated from a second library. The expression of human MCP (CD46) in these mouse strains was characterized by immunohistochemistry, FACS, Western blotting and RT-PCR. No differences were detected in the isoform pattern or distribution among the three strains, although the expression level varied according to how many copies of the gene were integrated. The expression profile closely mimicked that observed in humans, including the same pattern of isoform expression as the donor. In addition, tissue-specific isoform expression in the kidney, salivary gland and brain paralleled that observed in man. The transgenic mice expressed low levels of MCP (CD46) on their E, in contrast to humans but in line with most other primates. These mice should be a useful tool to analyse tissue-specific expression, to establish animal models of infections and to characterize the role of MCP (CD46) in reproduction.

Original languageEnglish (US)
Pages (from-to)180-189
Number of pages10
JournalClinical and Experimental Immunology
Volume124
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Complement system
  • MCP (CD46) transgenic mice
  • Measles virus receptor
  • Membrane cofactor protein (MCP; CD46)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Kemper, C., Leung, M., Stephensen, C. B., Pinkert, C. A., Liszewski, M. K., Cattaneo, R., & Atkinson, J. P. (2001). Membrane cofactor protein (MCP; CD46) expression in transgenic mice. Clinical and Experimental Immunology, 124(2), 180-189. https://doi.org/10.1046/j.1365-2249.2001.01458.x