Melatonin- and temperature-related changes in adrenoceptor stimulation of canine saphenous veins

Research output: Contribution to journalArticle

Abstract

Experiments were designed to determine whether or not melatonin causes temperature-dependent changes in adrenoceptor sensitivity in canine saphenous veins. Dogs were housed at 22°C with a 12/12 light/dark cycle (5000 Kelvin) for two weeks. Saphenous veins were removed, cut into rings, and suspended for the study of isometric force in organ chambers. The endothelium was removed deliberately from some rings. After an equilibration period of 40 minutes, a maximal response to norepinephrine was obtained. Concentration-response curves were obtained to an alpha1-and alpha2-adrenergjc agonists in the absence and presence of melatonin (10-9 through 10-7 M) 37°C and 24°C. Melatonin differentially affected alpha adrenoceptors: inhibiting at 10-6 M contractions to an alpha2-agonist and augmenting at 10-9 M an alpha1-agonist in rings with but not without endothelium. These effects were not temperature-dependent. These results suggest that melatonin may dose-dependently and differentially modify sensitivity of alpha- adrenoceptors through endothelium-dependent mechanisms in canine saphenous veins.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

saphenous vein
Saphenous Vein
adrenergic receptors
melatonin
Melatonin
Adrenergic Receptors
Canidae
endothelium
agonists
Endothelium
Temperature
dogs
temperature
Photoperiod
norepinephrine
Norepinephrine
photoperiod
Dogs
dosage
Experiments

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Melatonin- and temperature-related changes in adrenoceptor stimulation of canine saphenous veins. / Rud, K. S.; Miller, Virginia M.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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